| Objective:To explore the level of serum 3-nitrotyrosine(3-NT)in patients with type 2 diabetes and coronary heart disease and analyze its influencing factors.Methods:In this study,52 patients with type 2 diabetes mellitus(T2DM)combined with coronary heart disease(CHD)who were hospitalized in the endocrinology and cardiovascular medicine departments of our hospital from November 2021 to December 2022 were selected as the observation group(OB group),47 patients with T2 DM alone were selected as the T2 DM group(DM group),48 patients with CHD alone were selected as the CHD group(CHD group),and 50 healthy physical examination patients at the same time were selected as the control group(NC group).General information of the subjects was collected.Blood specimens were collected and subjected to routine biochemical tests and serum 3-NT levels were measured by ELISA.Cardiac ultrasonography was performed on all subjects.The SPSS 25.0statistical software was used to compare the indicators between the four groups and to analyze the correlation between 3-NT and the indicators and the independent influencing factors of 3-NT.Results:1.Comparison of clinical data among the four groups.Compared with the NC group and CHD group,the SBP level was significantly higher in the OB group(P<0.05);the BMI level was higher in the DM group,CHD group and OB group compared with the NC group(P<0.05).2.Comparison of routine blood biochemical indexes among the four groups.Compared with the NC group,the TG level gradually increased in the DM,CHD and OB groups,with the highest level in the OB group(P<0.05);compared with the NC and CHD groups,the FBG,Hb A1 c,FINS and HOMA-IR levels were significantly higher in the DM and OB groups(P<0.05).3.Comparison of serum 3-NT levels among the four groups.The serum 3-NT concentrations were(73.23±12.11)ng/m L,(102.31±11.78)ng/m L,(110.99±28.60)ng/m L,and(159.36±30.76)ng/m L in the NC,DM,CHD,and OB groups,respectively,compared with the NC group,the serum 3-NT levels gradually increased compared with the NC group,with the highest in the OB group(P<0.05),in which there was no statistically significant difference between the DM group and the CHD group in terms of 3-NT levels(P<0.05).4.Comparison of cardiac structure and function-related indexes among the four groups.The levels of LVEDD and LAD were higher and the levels of LVEF and E/A were lower in the OB group compared with the NC group(P < 0.05);the levels of LVEDD,LVESD and LAD were higher and the levels of LVEF and E/A were lower in the OB group compared with the DM group(P<0.05).5.Spearman correlation analysis of serum 3-NT and various indexes.Serum 3-NT levels were positively correlated with BMI,SBP,TG,FBG,Hb A1 c,FINS,HOMA-IR,LVEDD,LVESD,and LAD(r 0.24,0.23,0.63,0.42,0.54,0.39,0.48,0.19,0.22,0.30,P < 0.01,respectively),and negatively correlated with LVEF,and E/A were negatively correlated(r-0.40,-0.48,P<0.01).6.Multiple linear regression analysis of serum 3-NTThe results of multiple linear regression analysis showed that Hb A1 c,TG,and E/A were independent influences of serum 3-NT.Conclusion:1.Serum 3-NT levels were elevated in both type 2 diabetic patients and patients with coronary artery disease,and their levels were more significantly elevated in patients with type 2 diabetes combined with coronary artery disease.2.The changes in serum 3-NT levels correlated with lipid metabolism,blood glucose control and cardiac diastolic function indexes. |
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