Differential Expression Of Th1 And Th2 Cytokines In Tissues With HPV16/18 Infection And Different Degrees Of Cervical Lesions

OBJECTIVE: To investigate the immunological changes that occur locally in the cervix of patients with HPV16/18 infection and different degrees of cervical lesions.METHODS: A total of 120 cases pathologically diagnosed with cervical cancer(CC)after cervical biopsy or cervical conization were included in this study,and cervical squamous intraepithelial lesion(SIL)was the case group,which was then divided into HSIL group and LSIL group,and chronic cervicitis(CCS)was the control group.The high expression levels of IL-2,IFN-γ,IL-4 and IL-10 in the cervical tissue of each group were detected by immunohistochemistry.RESULTS:(1)With the progression of cervical lesions,the positive rate of HPV 16/18 subtypes gradually increased(P < 0.05),and the positive rate of other subtypes of HPV and the negative rate of HPV showed a decreasing trend.(2)With the escalation of cervical lesions,the high expression rates of IL-2 and IFN-γ decreased,and the high expression rates of IL-4 and IL-10 increased,and the differences were statistically significant for all indexes except IL-10(P < 0.05).Comparing between groups of each index,only the high expression rates of IFN-γ,IL-2and IL-4 had statistically significant differences between CC group and SIL and control group(P < 0.05);HSIL had significant differences compared with LSIL group only the high expression rate of IFN-γ decreased(P < 0.05).(3)There was a statistically significant difference between the HPV 16/18 subtype-positive group and the other HPV subtype-positive groups in terms of decreased IL-2 and IFN-γ high expression rates and increased IL-4 and IL-10 high expression rates(P < 0.05),and only decreased IFN-γ high expression rate and increased IL-4 high expression rate than the HPV-negative group(P <0.05).(4)Multi-factor logistic regression analysis showed that HPV 16/18 subtype positivity and IL-4 high expression were independent risk factors for the development of cervical lesions,and IL-2 high expression was its protective factor factor(P <0.05).Conclusions: 1.HPV16/18 subtype positivity is positively associated with the development of cervical cancer and there is an immune type shift in the local tissue of the cervix;2.There is also a decrease in Th1 cytokines and an increase in Th2 cytokines in the local tissue of the cervix as the degree of cervical lesions increases,with an immune shift in the direction of Th2.