Design And Application Research Of The Sweat Glucose Sensing Patch Based On Microfluidic Technology

There are a large number of biological markers in the sweat,such as lactic acid,urea,sodium and potassium,as well as antimicrobial peptides of tissue proteins and enzyme statin.In addition to the basic functions of regulating human body temperature and protecting skin tissue,the rich physiological information contained in sweat is also an unutilized human health information resource.Through the detection and analysis of sweat,the potential diseases of diabetes,calorie combustion value,electrolyte imbalance degree and other physical signs can be detected.At the same time,compared with the blood collection with puncture injury,the collection of sweat is more convenient,noninvasive,and can be carried out in real time outside the body,reducing the wound infection,and lower the aseptic requirements of the external environment during the collection.With the continuous progress of science and technology,sweat is expected to become a more ideal and safe human health monitoring information database than the traditional blood sampling,and the sweat sensors that can selectively detect the biological solutes in sweat have also begun to be gradually applied to the medical system.Therefore,exploring how to accurately and accurately obtain health information from sweat has become a hot issue in recent years.Based on the characteristics of less perspiration and more difficult to collect,the advantages of microfluidic technology in sweat collection have gradually emerged.The microfluidic patch has a trace of storage space,and the flexibility of the preparation material makes it flexible in the condition of non-toxic and harmless to human skin,with convenience to use and comfort to wear.With the development of wearable sensing technology in recent years,sweat sensors based on microfluidic technology can detect sweat in situ with fewer samples,and display the detection results through mobile terminals.Due to its convenient and noninvasive characteristics,it has received wide attention in the field of family medicine and disease risk prediction.The research contents of this paper mainly include:1.Based on the current microfluidic sweat collection patch that can be detected in situ and has timing needs to be processed and prepared by lithography technology,which has high production cost and processing time.In this paper,a low-cost microfluidic sweat collection patch for double-sided tape and transparent polyimide film is designed,which eliminates the steps of template making and reprinting,and reduces the production cost and process.The patch is composed of five layer paste,respectively on the top layer,spiracle valve layer,sweat flow layer,bottom layer and adhesive layer,formed four circular collection pool for storing sweat,sweat flow layer set the primary blasting valve used to control sweat flow,air valve layer set secondary blasting valve used to balance the patch inside and outside the pressure.Through in vitro simulation injection experiments,the results show that the sweat can be filled with four collection pools in time order,and the microfluidic-controlled sweat collection patch has good sealing,no leakage phenomenon,good flexibility and adhesion,and can better fit with human skin.2.For the analysis and monitoring of the collected sweat.A color marker with GOD attached were placed in each collection pool as the sensing layer,yielding a microfluidic sensing patch capable of sweat collection,storage and detection.The color marker can react with the glucose in sweat.By quantified the RGB value of color change and comparing with the standard color card,the corresponding sweat glucose value is obtained.The microfluidic sensing patch is highly selective for glucose and is not affected by other substances in sweat.Through human experiments,it was proved that the microfluidic sensing patch could get better sweat glucose detection results for different individuals.At the same time,the blood glucose change trend detected by commercial blood glucose meter was consistent with the change trend of sweat glucose detected by the patch,which proved the accuracy of the detection results.3.The rise and fall of human blood glucose can reflect the human health information to some extent,and the cause of the sudden rise and sudden drop of blood glucose may be other undiscovered diseases.Due to the existing microfluidic patches are difficult to completely discharge residual sweat after the measurement and the objects to be attached to the sensor,which cannot be used twice,the frequent replacement of new patches not only brings inconvenience to use but also increases the detection cost.Therefore,a microfluidic sweat glucose monitoring patch is designed to collect human sweat for a long time and detect it.By increasing the use time of a single patch,it can achieve the effect of detecting the fluctuation of human sweat glucose in a long time.By designing and exploring the structure of microfluidic channels,the influence of the inner walls of different hydrophilic channels on the sweat collection ensured the orderly collection of old and new sweat,so as to regulate the time of sweat collection.The microfluidic sweat glucose monitoring patch was collected for about 20-30 min.By comparing the value of sweat glucose at two adjacent time intervals,the information of human glucose or hypoglycemic was obtained.Due to individual or environmental differences,the collection time will fluctuate somewhat,but there is a significant increase compared with the existing microfluidic patches.It has good flexibility and can better fit with the human skin,and has good light transmittance,which reduces the measurement error brought by the color extraction process,and makes the detection results more accurate.