Characterization Of Intestinal Flora In Han And Mongolian Patients With Cerebral Infarction

Objective: In this study,16 S r DNA sequencing technology was used to analyze intestinal flora in feces of cerebral infarction patients and healthy people,and to screen out intestinal flora related to cerebral infarction.At the same time,intestinal flora of feces of cerebral infarction patients of Mongolian and Han nationality was analyzed to find the characteristic difference of intestinal flora of cerebral infarction patients of Mongolian and Han nationality.In this paper,different bacterial genera of intestinal microflora in Mongolian and Han patients with cerebral infarction were analyzed,providing theoretical basis for exploring the mechanism and therapeutic targets of cerebral infarction in Mongolian and Han nationalities.Methods: In this study,31 cerebral infarction patients(cerebral infarction group)and 11 healthy people(healthy control group)were collected from February 1,2022 to August 31,2022,including 11 Mongolian cerebral infarction patients(Mongolian cerebral infarction group)and 20 Han cerebral infarction patients(Han cerebral infarction group).The healthy population came from the physical examination center of the hospital at the same time,and all subjects signed informed consent.General clinical characteristics and blood biochemical index results of all subjects were collected.Fresh feces of the subjects were collected and stored in a-80℃refrigerator within 2 hours.16 S r DNA gene sequencing was performed on fecal specimens by Beijing Nuohe Zhiyuan Technology Co.,LTD.Based on the sequencing results,species difference analysis,Alpha diversity analysis and Beta diversity analysis were performed on the samples.The differential flora was screened out by LEf Se analysis and the bacterial flora function was predicted by PICRUSt2 software.Results:1.There was no significant difference in α diversity between the cerebral infarction group and the healthy control group,and between the Mongolian and Han groups(P>0.05).2.There were significant differences in β diversity between cerebral infarction group and healthy control group,and between Mongolian and Han cerebral infarction group(P<0.05).3.At the phylum level,compared with the healthy control group,the abundance of Bacteroidetes and Actinobacteriota increased in the cerebral infarction group,while the abundance of Firmicutes and Proteobacteria decreased,with no statistical significance(P>0.05).There were statistical differences between Mongolian and Han cerebral infarction groups in Euryarchaeota and Acidobacteriota(P < 0.05),and Eurycoarchaea only existed in Mongolian cerebral infarction group.4.LEf Se analysis results showed that,at the genus level,the abundances of Alistipes,Parabacteroides,Eubacterium＿coprostanoligenes＿group,Parasutterella and Coprobacter were significantly increased in the cerebral infarction group.The abundance of Faecalibacterium,Ralstonia,Streptococcus,Holdemanella and Subdoligranulum in the cerebral infarction group decreased(P <0.05).Compared with Han cerebral infarction group,the abundance of Faecalibacterium,Escherichia＿Shigella,Bifidobacterium,Lachnoclostridium and Dialister in Mongolian cerebral infarction group was higher.The abundance of Parabacteroides,Ruminococcys＿torques＿group,Muribaculaceae,Megamonas and Parabacteroides＿merdaewas lower(P < 0.05).5.The prediction of bacterial community gene function showed that compared with the healthy control group,the cerebral infarction group showed higher activity in the metabolism of glycerol phosphodiester phosphodiesterase,sialyl acetylesterase and other proteins(P<0.05).Compared with the Han cerebral infarction group,the bacteria in the Mongolian cerebral infarction group showed higher activity in the metabolism of pyruvate formate lyase,polysaccharide transport system permeability enzyme and other proteins(P <0.05).Conclusion: 1.Compared with healthy people,the composition and function of intestinal flora in patients with cerebral infarction were changed,and Alistipes and Parabacteroides were significantly enriched in patients with cerebral infarction,suggesting that intestinal flora may be involved in the occurrence and development of cerebral infarction.Regulating the imbalance of intestinal flora may be a new method to prevent and treat cerebral infarction.2.The abundance of Faecalibacterium and Escherichia＿Shigellain Mongolian patients with cerebral infarction is higher than that in Han patients with cerebral infarction,and there are differences in the metabolic pathway of bacterial flora between Mongolian and Han patients with cerebral infarction,which provides a lot of effective information for the subsequent exploration of the occurrence mechanism of cerebral infarction between Mongolian and Han,and also provides new ideas for effective prevention and treatment of cerebral infarction between different ethnic groups.It is worth further study.