| Research purposes:Based on the literature mining of traditional Chinese medicines in the network database for the treatment of chronic cholecystitis with hepatobiliary humid fever,the medication rules of chronic cholecystitis with hepatobiliary humid fever were explored,and data and theoretical basis were provided for clinical medication.Network pharmacology was used to predict the mechanism of action of core drug groups in the treatment of chronic cholecystitis from the molecular level,which provided a basis for follow-up research.Research methods1.CNKI Database(CNKI),Weipu Database(VIP)and Wanfang Database(Wanfang)were used as data sources to conduct data mining and analysis of traditional Chinese medicine prescriptions for the treatment of chronic cholecystitis with hepatobiliary humid fever since the establishment of the database until December 31,2021.After standardizing drugs using EXCEL,the Chinese medicine inheritance assistance platform was used to process the frequency,four qi,five flavors,efficacy,and meridian statistics of the drugs,and the SPSS Model and SPSS Statistics software were used to analyze the association rules and systematic clustering analysis of high-frequency drugs,and the core drug groups and new combinations were excavated.2.Using the network pharmacology as a method,using the TCMSP and STRING as a database to screen the effective compounds and corresponding component targets of the core drug groups of the data mining part,screening the disease targets of chronic cholecystitis(CC)through the Gene Cards database,standardizing the drug targets,using the Venny2.1platform to obtain the intersection targets of the core drug group for the treatment of chronic cholecystitis,mapping them with various traditional Chinese medicine components,and using cytoscape3.7.2software." Traditional Chinese Medicine-Disease-Target" network diagram,import intersection targets in STRING database,obtain PPI network diagram and topology analysis results,and beautify the PPI network diagram.The Matescape software was used to perform GO analysis and KEGG analysis of potential targets,predict the action pathway of the core drug group in the treatment of chronic cholecystitis,and visualize it.Results1.Obtained 254 prescriptions of traditional Chinese medicine for the treatment of chronic cholecystitis of hepatobiliary humid fever,and used frequency statistics to obtain a total of 129 flavors of traditional Chinese medicine,and 25 flavors of high-frequency drugs with a frequency of ≥ 30 times,and the top ten drugs were chaihu,scutellaria baicalensis,white peony,licorice,money grass,rhubarb,tulip,Yin Chen,Banxia,and Sichuan neem.The most used in the statistics of drug four qi is cold medicine,the most used in the statistics of drug five flavor is bitter medicine,the statistics of drug use are mainly shown to be spleen,liver,lung,stomach,and bile meridian,and the drug efficacy statistics are mainly water and moisture rejuvenation,heat clearing,deficiency replenishment,qi management,blood circulation and stasis.2.The results of the analysis of drug association rules showed that "Chaihu-Banxia-Shuangqin-Rhubarb" was the core drug group with the highest correlation,and it was a common drug group for the clinical treatment of chronic cholecystitis with hepatobiliary humid fever.3.Cluster analysis showed that high-frequency drugs could be clustered into seven new drug combinations,the first category was psyllium,zeppelin,gentian grass,gardenia;The second category is rhubarb,citrus fruit,skullcap,and banxia;The third category is magnolia and tangerine peel;The fourth category is chicken inner gold and knotweed;The fifth category is Sichuan neem,yanhuso,money grass,and tulip;The sixth category is saihu,white peony,licorice,poria,and baiju;The seventh category is citrus husk,dandelion,woody fragrance,and yin chen.4.Through the method of network pharmacology,the mechanism of action of the core group drug "Chaihu-Banxia-Scutellaria baicalensis-Rhubarb" in the treatment of chronic cholecystitis was predicted,and a total of 61 effective compounds,234 component targets,680 disease targets,and 60 drug-disease intersection targets were obtained.The key compounds are quercetin,baicalein,β-sitosterol,kaempferol,baicalein;The core targets of PPI topology analysis showed that the core targets were AKT1,TP53,VEGFA,IL6 and EGFR.GO enrichment analysis mainly involves negative regulation of cell population proliferation,positive regulation of cell migration,positive regulation of phosphorylation,response to inorganic substances,and regulation of epithelial cell proliferation.KEGG is mainly enriched in cancer pathway,NF-k B signaling pathway,hippo signaling pathway,HIF 1signaling pathway and other signaling pathways.Conclusion1.Based on data mining drugs,clinical use of water relusion,heat clearing,deficiency replenishment,qi management,blood circulation and stasis drugs for hepatobiliary humid fever chronic cholecystitis.Most of the drugs are bitter and spicy medicines,in order to seek their dry and wet qi work;The properties of the drug are mostly cold and warm,cold and temperature go hand in hand to prevent bitter cold;Drugs are mostly attributed to the spleen and liver meridians,in order to help the movement of the spleen and the ability of the liver to drain.The analysis of association rules showed that the drug group "Chaihu-Banxia-Shuangqin-Rhubarb" had the highest correlation,which was the core drug group,and the combination of the four drugs played the effect of clearing heat and moisture,and relieving choleretic,which was basically consistent with its treatment principles.2.It was predicted that the core drug group "Chaihu-Banxia-Scutellaria baicalensis-Rhubarb" in the treatment of chronic cholecystitis would exert its effect on targets such as quercetin,baicalein,β-sitosterol,kaempferol and baicalein through cancer pathway,NF-k B signaling pathway,hippo signaling pathway,and HIF 1signaling pathway. |
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