Expreession And Purification Human Recombinant Alpha A Interferon (IFN) In E. Coli And Construction Of Human Necrosis Factor-IFN Alpha, IFN Gamma-Alpha A Fusion Protein Using Oligonucleotide Directed Deletion Mutagenesis

IntroductionInterferons(IFNs) are very important cellular factors with several biologic functions. In 1957, IFNs were described a groupe of protein, produced by cells in response to virus infection, which could inhibit the growth of a wide range of viruses. Now it became clear that in addition to inhibit virus replication, IFNs have many important roles, such as regulating immune response, inhibiting tumor growth, resisting cell differentiation and showing a apparent synergic effect with several lymphokines. The studies of IFNs have been become a important part in the field of molecular biology, molecular immunology, molecular virology, Oncology, clinical service, and so on.IFNs are classified into three types alpha, beta and gamma, accoding to their antigenity. Three type IFNs came from mainly leucocyte, fibroblasts and lymphocytes individual. The IFNs are produced when cells are tarched with inducers. Many substances are inducers of IFNs, such as viruses, especialy double strand RNA virus, viruses specific antigen, polyclonal mutagen, metabolism of microbe, Poly I.C. and so on. There is a significant low amounts of natural IFNs in the cell, so it is very difficult to obtaine IFNs, especially pure IFNs, which not only hindered the study of IFNs, but also limited their clinical utilization. This situation changes dramatically when the human IFNs gene were cloned successfully, the cloning of the IFN genes...