| Octacosanol has significant antifatigue function. The extraction and purification of octacosanol from rice bran by SF-COa extraction, transesterification and MD were studied in this dissertation. Additionally, the antifatigue function of octacosanol extract was assessed, and a microencapsulated octacosanol was developed. The results were summarized as follows:1. A method for quantitative analysis of octacosanol by GC has been established. The samples were determined in HP-5 column, and TPB was used as the internal standard. The retention times of TPB, octacosanol and triacontanol were 8.28min, 10.09min and ll.Slmin respectively. The calibration curve of octacosanol was linear over a range from 200 u g/ml to 900 u g/ml with a correlation coefficients of 0.992, and the mean coefficient of variation for octacosanol was 0.72%.2. The solubility parameter theory was applied to evaluate solubility of rice bran wax and rice bran oil in SF-COi for first time. It was indicated that the oil was easier to be extracted by SF-CCh-Effects of particle size, pre-treatment by solvents, extracting pressure, extracting temperature, and extracting time on the extraction of wax and oil from rice bran by SF-CO2 were studied. The results of orthogonal experiment using rice bran with particle size of 0.18-0.86mm as material showed that the optimum parameters of wax from rice bran by SF-CO2 extraction were: extracting pressure SOMPa, extracting temperature 40 ℃, extracting time SOmin; The optimum parameters of oil from rice bran by SFE were: extracting pressure 50MPa, extracting temperature 40℃, extracting time 30min. Under the above conditions the yields of wax and oil reached 4.25% and 16.70% respectively. The wax and oil had higher yield and better sensory quality by SF-CO2 extraction in comparison with by n-hexane extraction, but the compositions of extracts were similar.3. Effects of different solvents on the refining of rice bran wax were studied. The results indicated that the composition, yield, melting point and sensory quality of refined rice bran wax were different. Acetyl acetate was a suitable refining solvent, and the wax yield and the octaconsanol content were the highest, which reached 57.8% and 12.5% respectively.4. Effects of solvent, reaction time, ration of wax to solvent, kind and concentrate of catalyst, reaction degree and extracting time on the extraction of higher aliphatic alcohol mixtures from refined rice bran wax by transesterification were studied. The results of orthogonal experiment showed that the yield of higher aliphatic alcohol mixture and octacosanol content were the highest when ration of wax to n-butynol was 1:12 (w/v), catalyzed by 0.2% KOH for 4h and extracted for 6h by acetone. Transesterification was beneficial to surrounding protection in comparison with saponification.5. Higher aliphatic alcohol mixtures were purified by MD to increase octacosanol content. Influence of distilling temperature and distilling vacuum degree on the purification were studied systematically for first time. The results of DORRD showed that optimum distilling temperature was 176癈, optimum distilling vacuum degree was 1.3Torr in the first MD, and the octacosanol content increased to 28.2%. The optimum distilling vacuum degree was O.STorr under 150癈 in the second MD,and the octacosanol content increased to 37.6%.6. Effects of octacosanol extract on behavior and biochemical index of swimming mice were examined. The increase of sport stamina was observed, in the meanwhile, some biochemical index also have been improved. Octacosanol extract would be considered to have an antifatigue bioactivity.7. A novel functional additive was developed by ME using acacia gum, P-HP-CD and maltose (1:1:2) as shell materials and octacosanol as core material. The optimum parameters of preparing stable emulsifying liquid for spray drying were: ratio of core material to shell material to Tween 80 was 1:10:5 (w:w:v), emulsifying temperature was 80℃, emulsifying time was 6h. The microcapsule produ...
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