A Study For Enterosoluble Microcapsules Of Lumbrokinase Digest

Lumbrokinase is a proteinase separated from a coarse abstract of earthworm protein,which has the activity of both kinase and cellulase. It possesses the actions of fibrillolysis,anticoagulation,andprotection to brain anemia.lt is used to cure and prevent brainstorm caused by brain anemia,coronary heart disease,diabetes, and hyperfibrinogenemia disease in clinic.Studies for oral preparation of protein and polypeptide drugs is an important developing direction of medicine research with biotechnology.This study is based on lumbrokinase as the material and polyacrylic acid resin as the carrier to study the enterosoluble micrones preparation of lumbrokinase.when soluent is ethanol or acetone,microcapsutes are not made successully in soluent-volatilizing way.Instudy of the preparation of lumbrokinase enterosoluble microcapsules a mixing solvent(named as Solvent S)is invented.Solvent S can dissolve Eudragit II and Eudragit III,and is not mix-soluble with water.When lumbrokinase water solution is took as the dispersive phase,Eudragit III solution in Solvent Sis took as the continuous phase,and mono-stearic glyceride is took as the emulsifying agent,a very stable W/O emulsion canform.A kind of microcapsules has been successfully prepared from mis emulsion by the spray-drying method. The drug -releasing ration is 2.2%by shacking for 2h in 0.1NHCL,and the ration is 99.6% by shacking for 2h in man-mand enteric juice. The pharmacokinetics of enterosoluble microcapsules is studied in dogs.Result is F% equals 122%compazing with the enterosoluble capsules of lumbrokinase,and between the groups,there is obvious difference (P<0.05).The study find the enterosoluble microcapsules inhibite obviously formation of dosage.This study first has found a kind of mixing solvent. And when lumbrokinase water solution is took as the dispersive phase,Eudragit solution in Solution S is took as the continuous,and mono-stearic glyceride is took as the emulsifying agent, a very stable W/O emulsion can form.The enterosoluble microcapsule has been successfully prepared from this emulsion.Second . In the evaluation for the lumbrokinase enterosolublemicrocapsules , I is drawn out to the lable the drug .By the means of radiation intensity measuring ,the determination problem of drug release has been successfully resolved . This is a beneficial explorarion for the research of oral enterosoluble microcapsule of the protein and polypeptide drugs.