| Syringa pubescens Turcz. is a kind of shrub with a wide distribution in China. Although the medical use of its plant has not been officially developed to date, its flowers have long been used to treat chronic hepatitis, enteritis, cancer of the esophagus, cirrhosis, and cancer of the liver among the people, and its toxicity is far below the natural level. So it may be an active potential medicine for the treatment and protection against hepttic fibrosis and carcinoma.In this thesis, we examined the chemical constituents of this plant. Three secoiridoid glucosides, oleuropein(l), 10-hydroxy oleuropein (2), oleoside-11- methyl ester(3) and 2-(3,4-dihydroxyphenyl) ethanol (4) were isolated by column chromatography on silica gel and TLC. Oleuropein was also separated from the secondary metabolite of the callus culture via silica gel chromatography and prep. HPLC, and its chemical structure elucidated on the basis of spectral analysis. A new method of RP-HPLC was established for the analysis of the contents of oleuropein, which is the main effective constituent. The contents of oleuropein in different positions of Syringa pubescens Turcz. and in the secondary metabolite of cell culture on the different culture conditions and in the products of chaperonage epiphyte culture were determined by the same RP-HPLC method. After reflux in 75%C2H5OH, the content of oleuropein from the flower of this plant was 14.88 mg/g, the root was7.21 mg/g; the leaf was 6.09 mg/g; the stem was 4.19 mg/g. The experiment result showed that the highest content of oleuropein was in the flower,On the basis of more than 300 times of experiments and more than 2000 test samples of data determination in this thesis, we gained the achievements in the following:1. The establishment of callus culture system and the suspension cell culture system of Syringa pubescens Turcz.Oleuropein synthesis in callus cultures of Syringa pubescens Turcz. was first described. Some types of calli were induced from the cotyledon, the spire, and the caulicle of Syringa pubescens Turcz. The influences of callus induction rate and growth form of different calli were examined on various kinds of culture mediums, plant growth regulators, kinds of explants. Growth curves of the cullus culture and the suspension cell culture were determined. The callus culture system and the suspension cell culture system of Syringa pubescens Turcz. were established. The callus induction rates were compared as: cotyledon> spire> caulicle.2. The establishment of optimum culture conditions.The effects of carbon sources, nitrogen sources, plant growth regulaters, pH, elicitor, culture medium, inoculum densities, subculture periods and culture conditions such as temperature and illumination time on bio-synthesis of oleuropein and callus growth were systematically studied in order to carry out the optimum culture conditions. By means of uniform design, the influences of components of medium and plantgrowth regulators on callus growth and bio-synthesis of oleuropein were investigated and optimized. A better result was found through colligation and comparison, i.e. (mg/L) MgSO4370, KH2PO4 255, KNO3 1805, NH4NO3 2145, CaCl2 396, thiamma-HCl 1.2, myo-Inositol 115, H3BO3 9.30, MnSO4 20.28, ZnSO4 10.32, NaMoO4 0.288, CuSO4 0.0275, CoCl2 0.0263, KI 0.789, Na2EDTA 33.570, FeSO4 23.63, KT 0.80, 2,4-D 3.30, NAA1.05; sucrose 30g/L; light time 1 Ohr/d; temperature 25 ; pH5.5; inoculum densities 20g/L; subculture period 35-42 days and opportune elicitors such as UV, SA and presume precursor (I) . The content of oleuropein from the culture callus reached 1.40 mg/g .3. The discovery of chaperonage epiphyte of synthesis oleuropein The chaperonage epiphyte was discovered from the root of Syringa pubescens Turcz. and cultured on LS or CDA medium. The oleuropein in existence from the cultured metabolites of the chaperonage epiphyte was confirmed by RP-HPLC. The initial study on culture medium, culture conditions and subculture period was carried out. Because the characteristics of chaperonage...
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