Characterization And Quality Control Of Multi-origin Chinese Drugs

To clear up the confusion that exists in the usage of Multi-origin ChineseDrugs is one of the key parts of the standardization of Traditional ChineseMedicines (TCMs). In this dissertation, some different plants used as the samedrug (XIXIN) were studied by modern characteristic methods to reveal thedifference of those plants in chemical composition and then to define a uniqueplant as the origin of XIXIN.Gas chromatography/qudrapole mass spectrometry (GC/qMS) and Gaschromatography/time-of-flight mass spectrometry (GC/TOF-MS) were applied toanalyse the essential oils from some different plants, as well as the retention indexmethod for peak identification. The oil chemical composition from different plantsis remarkably different. In addition, the important chemical information of somewild plants listed as endangered species was obtained. The analgesic andanti-inflammatory effects of the essential oils from A. chinense Franch and A.himalaicum were reported for the first time.The fragmentation mechanisms of bisepoxy lignans were investigated byelectrospray/atmospheric pressure chemical ionization tandem mass spectrometry.Furthermore, an HPLC/MRM/MS/MS method was developed for identification ofsesamin and asarinin in Asarum L. These two compounds were founded for thefirst time in four species of Asarum at very low concentration. Besides, theobtained results indicated that the isomerism of secondary metabolites is related toplant origins. In order to verify the hypothetical fragmentation mechanisms,L-Planinin, a bisepoxy lignan in Zanthoxylum planispinum, was checked by thedeveloped HPLC/MRM/MS/MS method. Argentation reversed-phase high-performance liquid chromatographic method(Ag-RP-HPLC) was developed for analytical and preparative separation ofunsaturated aliphatic acid amides. A new compound and three known compoundswere isolated from Asarum forbesii Maxim. In comparison to argentationnormal-phase liquid chromatography (Ag-NP-LC), the developed method is rapid,simple, efficient, and inexpensive. The separation of complex mixtures can beachieved using gradient elution of silver ion concentration. A hypertoxic substance, safrole, was discarded from hexane extracts ofAsarum heterotropoides by preparative HPLC. The acute toxicity of the remainedextract is decreased while the anti-inflammatory activity is enhanced. On the basis of the chemical composition analysis, A. heterotropoides wasdefined as the origin plant of XIXIN. The multi-mode chromatographicfingerprints method of XIXIN was developed for quality control. The similaritybetween chromatographic fingerprints of standard sample and nineteen testsamples was compared, and the similarity index was put forward based on thevector.