The systemic and comprehensive chemical research from from Patrinia villosa Juss was carried out, and 42 compounds were obtained and 39 of them were identified, and the rapid preparation platforms of some compounds have been established by highspeed counter-current chromatography and the preparation protocol of the active fraction of the famous Chinese medicine formular YiyiFuziBaijiangSan has also been established. In the meanwhile, the antioxidant and anticancer activities of the obtained compounds from the plant and the active fraction were all evaluated, and the quality standard of the active fraction was also established.
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