Preparation Technology And Characteristic Research On Puerarin Phospholipids Complex In Vitro And In Vivo

In present study, puerarin phospholipids complex (PPC) was prepared by co-precipitation to improve oral absorption of puerarin according to its low solubility and low oral bioavailability. The research focused on the formation mechanism of PPC and its physicochemical and biological characteristics on the basis of optimizing the preparation conditions. The PPC was also prepared by supercritical fluid precipitation (SFP) technique and the physicochemical characteristics of PPC prepared by four different methods were compared. Five main sections were included in this paper:1,The determination of the solubility of puerarin (Pur) , the component identification and phosphatidylcholine(PC) content determination of phospholipids:The results showed that Pur is slightly soluble in water. The component in phospholipids is identified by TLC in three developing solution with different polarity. A HPLC method was developed to determine the content of PC in phospholipids by using C₈ column and the content was 62.6%.2, The optimization of the reaction conditions and the preparation of PPC by co-precipitation:(1) New attempts were made to the research of the reaction conditions of PPC, and a reasonable criterion of accurate evaluation was established, by which such actors as solvent, temperature, the ratio of puerarin to phospholipids and the concentration of reactants were examined. The orthographic design was used in the optimization of reaction conditions and the best using absolute ethyl alcohol as solvent with a simple reaction condition was obtained through statistical analysis and proof test.(2) A UV method was established to determine the content of Pur in PPC accurately and simply.3,The research on the physicochemical characteristics, the structure confirmation and formation mechanism of PPC prepared by co-precipitation :(1) The microcosmic form of the PPC was observed by scanning electronic microscope (SEM), the results showed that the cascading flakes structure of the original Pur changed to structure entrapping by phospholipids-like materials with more uniform size. X-ray diffraction showed that the complex exhibited an amorphous characteristic and the crystal diffraction peaks...