Studies On The Relationship Between Phaeocytstis Globosa And Algae-lysis Bacteria

Phaeocytstis globosa, one of the harmful algal bloom(HAB) causing species, had recently caused red tide in the South-China and East-China sea area Since physical and chemical methods to prevent and treat P.globosa blooms didn't work well, the algae-lysis bacteria have been considered as a biological alternative.This study is composed of three parts. Firstly, the growth characteristic of P.globosa was investigated, also the complex heteromorphic life cycle of P.globosa was studied. Secondly, Two algae-lysis bacteria could kill P.globosa were isolated from seawater of ZhuHai, and 16SrDNA of these strains were identified by a sequence analysis, at At last, the fluorescence character of P.globosa and the algae-lysis bacteria were examined. The research lists as following:1. P.globosa can suffer from a large range of salinity, it can even survive at 0 psu salinity conditionswhich d. Furthermore, cell surface topography of different salinity was compared at 0 psu and 36 psu respectively by Atomic Force Microscope. The result indicated that the cell size of algae cultured at 0 psu was bigger than that of 36 psu at the corresponding period. Besides, the cell surface of P.globosa was rough and obviously dented.2. P.globosa has had a complex heteromorphic life cycle between free-living cells and colonies. The life cycle of P. globosa strain showed that the little cells colonies can could attach to each other by the amylose excreted by the cells and become one big colony. The colonies can could also dimidiate. In batch culture , Tthe colonies of P. globosa takes lived about 14 days, and they can could not be formed again after three generationsexceed .3.The algae-lysis bacteria Y01, Y04 were isolated from seawater,. bBoth of them...