Characteristic Research On The Wines From Geographic Origin Of Changli Using Three-Diminsional Fluorescence Spectroscopy And Electronic Tongue

Wine is one of the most complex alcoholic beverages and has very high value ofnutrition and health protection function.More and more consumers concern the objective andauthentic of wine,but the protection of wine is not enough,and the law and regulation aboutwine control of origin are not perfect.The quality of wine is poor in our country,and there aremany adulteration in market.In this situation,there needs the technique which should hesensitive,nondestructive,rapid,environmentally friendly to discriminate the adulterate wine,class wines,even the wine with differemt thegeographic origin.Three-dimensionalfluorescence spectroscopy and electronic tongue are selective,sensitive and non-destructivetechnique,and receiving the widespread attention and application.The objective of the present study was to assess wine from the geographic origin ofChangli County,supplied directly by the Huaxia Winemaking Company and YueqiannianWinemaking Company using three-dimensional fluorescence spectroscopy and electronictongue.In order to discriminate the wine from the geographic origin of Changli County,thethree-dimensional fluorescence spectroscopy and the electronic tongue spectroscopy of thewines with different variety,brewery and vintage were established.According to the result,ascientific method for identifying the wines quickly and reliable was established.The scientificbasis for the protection of wine with the geographic origin was provided,and the newtechnical method also can evaluated and analyze the whole characteristic of wines.In our study spectrofluorometer(F-7000,Hitachi)and Multi-frequency amplitude pulseelectronic tongue(MFP-ET)were used to analyze the wines from the geographic origin ofChangli County with different variety,brewery and vintage,the result as follow:1,Three-dimensional fluorescence spectroscopy was used to analyse the wine withdifferent variety and brewery.Three-dimensional fluorescence spectroscopy with different variety produced in 2004and 2005 had obviously different in the number of fluorescent peaks and the fluorescenceintensity.The wines produced in 2004 were study,and the result showed that Gamay had twofluorescent peaks at excitation wavelength 260nm and 335nm;Cabernet Sauvignon,Shpusi, Syrah and Merlot had three fluorescent peaks at excitation wavelength 263nm,290nm and329nm.The wines produced in 2005 were study,and the result showed that Gamay had twofluorescent peaks at excitation wavelength 260nm and 332nm;Syrah had two fluorescentpeaks at excitation wavelength 290nm and 329nm;Cabernet Sauvignon,Shpusi and Merlothad three fluorescent peaks at excitation wavelength 263nm,290nm and 329nm.Thefluorescence intensity was different with different variety.Three-dimensional fluorescence spectroscopy was applied to analyze the wine producedin 2005 with different brewery.The result showed that Cabernet Sauvignon and Merlot hadthree fluorescent peaks at excitation wavelength 263nm,290nm and 329nm,and thefluorescence intensity was obvious distinctness with different brewery.Three-dimensional fluorescence spectroscopy can discriminate the wines with differentvariety and brewery by means of the number of fluorescent peaks and the fluorescenceintensity.2,Three-dimensional fluorescence spectroscopy was used to analyse the wine ofCabernet Sauvignon with different vintage.Three-dimensional fluorescence spectroscopy was applied to analyze the wines ofCabernet Sauvignon with different vintage.The result showed that the number and theintensity of fluorescence peaks were different because of climate change every year.Thethree-dimensional fluorescence spectroscopy showed that Cabernet Sauvignon produced in1993 had two fluorescent peaks at excitation wavelength 296nm and 326nm;CabernetSauvignon produced in 1996 had one fluorescent peak at excitation wavelength 329nm;Cabernet Sauvignon produced in 2001,2003,2004 and 2005 had three fluorescent peaks atexcitation wavelength 260nm,29nm and 329nm.The fluorescence intensity was obviouslydifferent with different vintage,so three-dimensional fluorescence spectroscopy can be usedto discriminate wines with different vintage.3,Principal component analysis was used to analyze the three-dimensionalfluorescence spectroscopy of wines with different variety,brewery and vintage.Principal component analysis was applied to analyze the three-dimensional fluorescencespectroscopy of wines with different variety,brewery and vintage,and the principalcomponent PC1,PC2 and PC3 were extracted.The information quantity of PC1,PC2 andPC3 was 75 percent of the total variance,and the score map of PCA showed that the three-dimensional fluorescence spectroscopy could be applied to discriminate the wines withdifferent variety,brewery and vintage. 4,Multi-frequency amplitude pulse electronic tongue(MFP-ET)analyzed the winewith different variety,brewery and vintage from the geographic origin of ChangliCountyThe wines with different variety,brewery and vintage were analyzed using MFP-ETthrough a sensor array of platinum,gold,silver,titanium,palladium,cadmium,aluminumand cuprum electrodes under the mentioned three frequency segments of 1 Hz,10 Hz and 100Hz.Principal component analysis was used to process the data.The results showed that thewine could be successfully discriminated by combining together gold electrode in 10 Hz,silver electrode in 100 Hz,cadmium electrode in 1 Hz and titanium electrode in 100 Hz.MFP-ET based on selecting the appropriate sensor array which was composed of thedifferent non-modified metal electrodes and combining together the different frequencysegments of multifrequency large amplitude pulse voltammetry,and showed a promisingapplication in qualitative and quantitative analysis of wine.