| Anoetochilus roxburghii (Wall) Lindl. and A. formosanus, belonging to Orchidaceae, are rare medicinal plants. The most suitable root growth medium for A. formosanus was 0.125MS+1.61mg·L-1+1.50mg·L-1+2.20g·L-1 active carbon, and that for A. roxburghii was 0.36MS+3.00mg·L-1+1.50mg·L-1+0.80g·L-1 active carbon. After 60ds'culture on the root growth medium, the roots number of A. formosanus and A. roxburghii reached 2.33 and 2.45 respectively.A. formosanus and A. roxburghii were transplanted to flowerpots which filled with 5 kinds of culture substrate. The results showed that the most suitable substrate was humus soil:vermiculite (3:1). After 90ds'culture in the substrate, the average fresh weight, dry weight and height of A. formosanus were 1.197g,0.136g and 11.302cm, and that of A. roxburghii were 1.177g,0.131g and 10.856cm.Wild Anoetochilus plants are known to form a symbiotic relationship with fungi. Based on the characteristic, we cultured 43 strains of fungi from orchids together with seedlings of A. roxburghii and A. formosanus on agar medium for 2 months.10 strains could promote seedlings of A. formosanus growth and 8 strains could promote seedlings of A. roxburghii growth.To make sure the promotion activity of the fungi, we inoculated them to the roots of A. formosanus and A. roxburghii. After 90d, the average fresh weight and dry weight of A. formosanus inoculated with AR18 were increased by 15.08% and 19.403% respectively, compared with the non-inoculated ones, while the average fresh weight and dry weight of A. roxburghii inoculated with AR18 or AR14 were increased by 17.155%,22.584% and 15.736%,18.652% respectively, compared with the non-inoculated ones.The element of A. formosanus and A. roxburghii were measured by ICP. The results showed that K content was higher than other macro-elements, and the Mg content was the lowest. Fe content was the highest in the trace elements. Different fungi have different effect on the element contents of A. formosanus and A. roxburghii.The content of Kinsenoside and flavone of A. formosanus and A. roxburghii were detected by HPLC to study the effect of fungi on the secondary metabolite. The content of Kinsenoside in cultivated A. formosanus and A. roxburghii rising over time, and the inoculation of fungi increased the Kinsenoside content. The promotion effect of AR18 on A. roxburghii was more significant than on A. formosanus. The Kinsenoside content of the A. formosanus and A. roxburghii inoculated with AR18 were 1.97 and 1.24 times respectively as much as control at the 8th week. While, the promotion effect of AR14 on A. roxburghii was inferior to AR18, the Kinsenoside content of A. roxburghii inoculated with AR14 was 1.63 times as much as control at the 8th week.The kinds of flavonoid were different in A. formosanus and A. roxburghii, and it didn't change with fungi inoculation. Only Isorhamnetin-3-O-β-D-glucopyranoside was detected in cultivated A roxburghii, and the inoculation with AR14 could increase its content while AR18 first suppress then increase its content.4 flavonoid were deteced in cultivated A. formosanus and 2 of them could be characterized as Isorhamnetin-3-O-β-D-glucopyranoside and Quercetin. AR18 first suppress then increase the content of Isorhamnetin-3-O-β-D-glucopyranoside, while it increases the content of Quercetin all the time.
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