Dynamic Change Of Main Active Ingredients In A.roxburghii (Wall) Lindl And A.formosanus Hayata In Different Growth Stages

Anoectochilus formosanus is widely used in folk and it enjoys the laudatory name of" Medicine king ". Its chemical constituents and pharmacological activity have been wellknown gradually. Because of great excavation, the wild resource was in danger. Nowadays,adopting biotechnology is the main way to breed Anoectochilus formosanus. What thedifferences of activity component between tissue cultured species and wild species as well aswhether cultured species could take the place of uncultured species can not make conclusions.The dynamic changes of the main active ingredients in the Anoectochilus formosanus werestudied in this paper. Meanwhile the cumulative rules of active ingredients in artificialAnoectochilus formosanus were analysed and comparsion between two species wereresearched. The subject could provide an important theory for confirming best harvest time inindustrial production. Results were as follows:1. HPLC rapid test of flavonoid aglycones was established. Column was Agilent column(TC-C18(2)4.6×250mm,5μm). Column temperature was30℃and mobile carrier wasmethanol-water-phosphoric acid (59:41:0.3%). Flow rate was1.0mL/min, UV wavelengthwas370nm and injection volume was50μL. Two kinds of Anoectochilus formosanusflavonoid aglycones were detected simultaneously. Meanwhile, the separation accuracy washigh and the peak time was early by this method.2. Polysaccharides contents and flavonoids were rich in Anoectochilus formosanus. Theregulations of the contents of polysaccharide and total flavonoids which from cultivatingstage to transplanting stage were "double-peak curve"mode.Meanwhile, the trends of thecomposition change including stems and leaves were nearly same. The contents of Quercetin,kaempferol and isorhamnetin increased with the "single-peak curve" mode in Anoectochilusroxburghii. In A.formosanus Hayata, the contents of isorhamnetin increased with the"double-peak curve" mode, while another two were "single-peak curve" mode.3. The contents of Polysaccharide which cultivated5months were more than that of4months in cultivation stage. And A.formosanus Hayata was more than Anoectochilusroxburghii. In the transplant stage, the content of Polysaccharide in Anoectochilusroxburghii reached the maximum after5months, and the A.formosanus Hayata reached the maximum after4months.4. The total flavonoids contents of two Anoectochilus formosanus after cultivating5months were higher than that of4months in cultivation stage. In the transplant stage, thecontent of total flavonoids reached the maximum after4months (in early July).5. In rooting cultivation stage, the contents of flavonoides in two Anoectochilusformosanu were different. Quercetin was the most and isorhamnetin was more thankaempferol. The total flavonoids contents of two Anoectochilus formosanus after cultivating5months were higher than that of4months in cultivation stage. In the transplant stage, thecontent of total flavonoids reached the maximum after4months (in early July) and it wasmore than wild Anoectochilus roxburghii6. The Polysaccharide in stems was more than in leaves. For the Polysaccharide ofleaves, Anoectochilus roxburghii was more than A.formosanus Hayata. In rooting cultivationstage, the Polysaccharide of stems of A.formosanus Hayata was more than Anoectochilusroxburghii. After transplanting5months, the Polysaccharide of Anoectochilus roxburghii wasmore than A.formosanus Hayata. The total flavonoid was accumulated in the leaves and thecontents in stems were relatively small. What`s more, the total flavonoids of stem ofA.formosanus Hayata were more than Anoectochilus roxburghii.7. From the perspective of biological yield, after rooting cultivating5months, weight anddryingrate in two kinds of Anoectochilus formosanus were higher than4months. Intransplant stage, the results showed that transplanting could improve two kinds ofAnoectochilus formosanus solidscontent and Anoectochilus formosanus should betransplanted after4months.