Nutritional Mechanism And Relative Availability Of Iron Amino Acid Chelate

With the rapid development of intensive animal husbandzy and the gradually enhancement of protecting environment consciousness, iron amino acid chelate which is main representative of the third generation of iron additive is more and more important in animal nutrition. Four experiments were conducted to study the nutritional function mechanism and relative biological availability of iron amino acid chelate. In this study, rats were used as animal model, Fe-Lys and Fe-Gly were studied as representative of iron amino acid chelate.I Utilized the technique of perfusing ligated intestinal segment, in vivo, in situ combined with radioactive isotope tracing, iron absorption of iron amino acid chelate was studied by comparing with the compound of amino acid and FeCI2 in the same moles of chelate. 42 fifty day-old male Wistar rats fed four types diet (purified diet based on dried skim milk, corn starch and sucrose, purified diet supplemented with 250 mg/kg Cu, purified diet supplemented with 1% sodium phytate, and conventional diet based on corn and soybean) were used. The results indicated that: Iron concentration in spleen, liver and thighbone and hemoglobin of rats fed purified diet supplemented with 250 mg/kg Cu for 28 days was significantly lower than that of rats fed purified diet. The influence of purified diet and conventional diet on iron store of rats was significant. Lysine and glysine could increase the absorption of ferrous iron, rats could effectively absorb iron in Fe-Lys and Fe-Gly by comparing with the compound of amino acid and FeCl2 in the same moles of chelate. 1% sodium phytate in diet could significantly decrease iron absorption in FeCI2 and ferrous amino acid chelate, however, influence on the latter was less than the former. The effect of high Cu in diet on the iron absorption of FeCl2, Fe-Lys and Fe-Gly was not significant. The effect of factors in conventional diet that restrict iron absorption on iron of FeC 12 was stronger than that of Fe-Lys and Fe-Gly. According to the results of comparing with the compound of amino acid and FeCI2, and the ability resisting factors that disturb iron absorption, iron amino acid chelate was an excellent iron additive. In addition, the results showed that the technique of perfusing ligated intestinal segment in situ combined with radioactive isotope tracing was an ideal method to study trace element absorption.2 Iron absorption, transport and utilization of iron amino acid chelate by rats in normal physiological status was studied through comparing with the compound of amino acid and FeCI2 in the same of moles. The technique of directly perfusing stomach combined with radioactive isotope tracing was used in this study. 120 fourty day-old male Wistar rats werefed two diets: purified diet based on dried skim milk and corn starch and sucrose, and conventional diet based on corn and soybean. The results indicated that: (X) Lysine and glysine could increase absorption, transport and utilization of ferrous iron. ?Comparing with the compound of amino acid and FeCI2 in the same moles of chelate, iron-depleted rats could more easily absorb, transport and utilize iron in iron amino acid chelate. ?iron absorption, transport and utilization of iron amino acid chelate by rats, which was in well iron status, were better than that of FeCI2. ?The results obtained from this study indicated that iron amino acid chelate could be considered as an excellent iron additive. (~) Thougb investigating dynamic change of radioactive concentration in blood, red cell, serum globin and duodenum, and determining other conventional indexes, the technique of directly perfusing stomach combined with radioactive isotope tracing was an appropriate method to study iron absorption, transport and utilization by animals.3 Using the slope-ratio technique, an experiment was conducted with 39 thirty day-old male Wistar rats to estimate bioavailability of different iron sources. 3 X 4 factorial design was used in this experiment. Rats were allotted randomly to thirteen dietary treatments...