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Inheritance Of Resistance To SMV3 And Identification Of Molecular Markers Linked To Resistance Gene In Soybean

Posted on:2001-09-10Degree:DoctorType:Dissertation
Country:ChinaCandidate:C M ZhengFull Text:PDF
GTID:1103360002452456Subject:Crop Genetics and Breeding
Abstract/Summary:PDF Full Text Request
Soybean mosaic virus (SMV) occurs worldwide and is a major soybean disease in China, resulting in yield losses and seed-quality deterioration . Using resistant varieties is the most economic and effective method to control SMV. Northeast China is the main soybean production region in China and the virulence of SMV 3 is the strongest. It抯 very important to identify and study new resistant germplasms. We have screened resistant soybean germplasm by inoculation of SMV3 and investigated the genetic diversity of part of the resistant varieties with RAPD and morphological characters. We have demostrated the inheritance of resistence of 95-5383 by studying the progenies of the crosses of resisantxsusceptible . We have identified one RAPD marker closely linked to SMV resistance gene by BSA and converted it into SCAR marker. The RAPD marker have been mapped in soybean molecular linkage group by using a mapping populations. The main results are summarized as followings: 1. 347 soybean accessions have been identified for the resistance to SMV3 by artificially inoculation with SMV 3, a strong virulent strain in Northeast China. The results showed that 113 accessions were highly resistant to SMV3. account for 32.56%; 112 accessions were moderately resistant to SMV3. account for 32.27%; 122 accessions were susceptible to SMV3, account for 35.16%. Resistant accessions were mainly originated from Northeast China (Liaoning), Huang Huaihai areas (Shandong, Shanxi province and Beijing), U.S. and South Korea. There were few resistant accessions from South China in our evaluation. Symptoms are different in different varieties when inoculated with the same SMV strain, showing there are interaction between soybean varieties and SMV strains. Top necrosis symptoms were observed in introduced varieties from abroad, indicating symptoms may relate to geographical origin of the accessions. Some varieties with known resistance gene introduced from U.S. were identified for the resistance to SMV3 and the relationship of resistance gene between Chinese accessions and U.S. accessions was discussed. 2 One highly resistant soybean line (95-5383) was crossed with four susceptible soybean varieties/line (HB 1, Tiefeng2 1, Amsoy, Williams) and one resistant variety (P1486355) Their Fl and F2 individuals were identified for SMV resistance by inoculation with SMV3. The results showed that in the four crosses of resistantxsusceptible, Fl were susceptible and F2 populations segregated in a 1 resistant: 3 susceptible (mosaic and necrosis) ratio, indicating that 95-53 83 carries one recessive gene that confer resistance to SMV. There are segregations of susceptibility in F2 progenies from the cross of 95-5383x P1486355, indicating that the SMV resistance gene in 95-5383 is located at different loci from that in P14863 55. By bulked segregant analysis (BSA) in F2 progenies of 95-5383xHB1, we have identified one codominant RAPD marker OPNI 1980/1070 closely linked to SMV resistance gene. DNA fragment OPN1 1980 was amplified in 95-5383 and resistant bulk, OPN1 1)070 was amplified in HBI and susceptible bulk. OPN1 1980,10,0 was amplified in Fl. Identification of the markers in F2 plants of 95-5383xHB1 showed that the codominant marker OPN1 1930,10,0 is closely linked to the resistance gene in 95-5383, with genetic distance of 2.1cM. 3 Both fragments of OPNI ~ and OPNI 110,0?amplified from 95-5383 and HB 1. respectively, were purified and cloned into PGEM-T easy vector and sequenced.
Keywords/Search Tags:Soybean mosaic virus. Identification of resistance, Inheritance of resistance, Resistance gene. RAPD. SCAR
PDF Full Text Request
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