| An RFLP linkage map based on the recombinant inbred FIO lines (RILs) derived from a cross between a japonica cultivar Taichung 65 and an indica cultivar ARC 10313 was constructed. QTL mapping of antibiosis to Green Leafhopper(GLH), Nephotettix virescens Distant, was conducted and the genetic basis of the resistance was identified with near isogenic lines of Grh2 and Grh4 genes. Grh2 gene was transferred through backcrossing and CAPS markers assistant selection. In addition, QTLs controlling spikelet and pollen sterility, F2 sterility and heading date were also identified. The results are as follows:1 Construction of RFLP linkage mapTo exploit the genetic potential of rice, 125 recombinant inbred F10 lines (RILs) derived from a cross between & japonica cultivar Taichung 65 and an indica cultivar ARC 10313 were developed. As a first step, an RFLP linkage map based on the RILs was constructed. The RFLP map contained 113 well-dispersed RFLP makers. Total map length was 1462.4cM. Linkage arrangement of the RFLP markers was in good agreement with that of the previously constructed maps.2 QTL mapping of antibiosis to Green Leafhopper, N. virescens and identification of genetic basis of the resistanceGreen Leafhopper (GLH), is one of the major insect pests of rice in the temperate Asian rice region. Nine GLH resistance genes (Glhl-glh8, Glh} and 4 Green Rice Leafhopper (GRH), Nephotettix cincticeps Uhler, resistance genes (Grhl, Grh2, Grh3 and Grh4) have already been identified. In this paper, QTL for antibiosis to GLH were investigated on Chromosome 3, 5, 11 and 12. Among them, the QTLs nearXNpb292 on chromosome 12 for increasing GLH mortality was fromjaponica variety. Among the other three QTL from indica variety, two QTL on chromosome 3 and 11 were close to Grh2 , and Grh4 , respectively. Therefore, the resistance to GLH was studied with near isogenic lines of Grh2 and Grh4 derived from susceptible variety Kinmaze(japom'ca), and resistant variety DV85 (indica). The results revealed that these two GRH resistance genes interaction also expressed strong resistance to GLH. None of the 9 Glh genes reported was located on chromosome 3 or 11, but 2 QTL for resistance to GLH newly identified in ARC 10313 were detected on chromosome 3 and11 in this study, which may correspond to Grh2 and Grh4.3 Green rice leaf leafhopper, N. dncticeps resistance gene transferring through backcrossing and CAPS Markers assistant selectionGrh2, one of green rice leafhopper, N.dncticeps resistance genes was- located on chromosome 11 of resistant variety DV85 (indica). Taichung 65 is ajaponica cultivar with elite characters, but susceptible to green rice leafhopper. C189 and G1465 are two RFLP markers flanking Grh2 gene, which were transformed into CAPS markers in this study. Both phenotypic selection and CAPS marker assistant selection were conducted in BC6F2 population derived from the cross of Taichung65 and DV85 in order to pick out the important breeding materials with Taichung65 background and resistance to green rice leafhopper. The linkage distance was calculated with the molecular and phenotypic data, meanwhile the effect of the selection method was analyzed. Since the experimental methods in this paper, including rude extraction of DNA, CAPS primer design, and the method of CAPS marker assistant selection, were characterized by fast, easy, and high effective to operate, it can be applied broadly to the selection for various agronomic traits.4 Identification of QTLs controlling spikelet and pollen sterilityQuantitative trait locus (QTL) analysis has been carried out to identify genes conferring spikelet sterility and pollen sterility in rice. One hundred twenty five F10 RILs derived from the cross between ajaponica cultivar Taichung 65 and an indica cultivar ARC 10313 were developed. Sixty three recombinant inbreed F, (BF,) lines derived from backcrossing RILs to Taichung65 were used as a segregating population for QTL analysis. Three QTLs for F, spikelet sterility were detected on Chromosome 1, 7 and 11 usin...
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