| Mine soil-reclaimed is one of comprehensive key research field in the world. The area of red soils accounts for 20% of the total land in China, and has great potential for mineral resources and is main base of metal in China. Along with development of mining in this region, abnormity of heavy-metal endogenetic geochemistry change to heavy-metal inchoative geochemistry, which lead to accumulation and diffusion of heavy metal in mine region of red soil and great red soil area, serious degradation of soil caused by heavy metal pollution, and mine ecological system destroy. Many researches were done domestic-overseas for the sake of removing the soil environment negative-effect, accelerating the process of soil-reclaimed and slowening the contradiction between population and land. But most of studies were focus on the process of vegetation restoration and engineering technology of mine soil ecological system, rather than underground soil microbes rehabilitation, evolvement and effect on the whole ecological system of mine area. In the paper, a series of the field investigation, laboratory culture and pot experiments were conducted, to study microbial eco-characteristic and restoration in relation to soil-plant system of red soils reclaimed-mine. The main results were summarized as below:1. Soil microbes eco-characteristic in Lipu Copper mining reclaimed soil-endurance plant system were studied comparatively, Zhe Jiang Province. The results indicated that, the total quanlity of major soil microbes declined, of which the minesoils was decreased by 68.43%~80.32% in the top soil(0-20cm) compared with that of the non-minesoils. The proportion of bacteria and actinomyces in the amount microbes decreased, while that of fungi not obviously changed. The amount of major physiological group including ammonifiers, nitrogenfixing bacteria, celluosedecomposing microbes, aerobic nitrogen fixing bacteria and anaerobic nitrogen fixing bacteria all decreased. The activity of soil enzyme weakened which include invertase, urease, proteinase, acid phosphtase, catalase, polyphenol oxidase and peroxidase. Soil biochemical action impaired which ammonification, nitrification, nitrogen fixation and decomposition of cellulose. As to the soil basic respiration, the release amounts of CO2 decreased, all of which may led to the degradation of microbes activity in minesoils. Relations were analyzed between plants and soils. The results indicated that metal content of elements Elsholtzia haichowemis in sequence were:Cu>Zn>Pb>Cd. There was significant correlation in Cu content between plants and soils, and the next was in Zn content. The minesoils possessed obviously different mciobialfeatures: higher in microbial basal respiration strength, and microbial ecophysiological parameters Cmic/Corg and qCO2, but lower in micobial biomass compared with the non-minesoils. Biolog data showed that were changed, much more rapid in consuming carbon sources, but the lower in the ratio of utilization. All of which showed, soil microbial feature can as an effective index of soil-plant system in Cu mine area.2. Soil microbes eco-characteristic in Tiantai Pn, Zn mine reclaimed soil-endurance plant system were studied comparatively, Zhe Jiang Province. The results showed both total and soluble metal loadings in these soils increased with proximity to the tailing mine reserve. Indictors of soil microbial features varied significantly because of heavy metal contamination stress. Soil microbial biomass and viable population size (plant counts) were negatively affected by the elevated metal levels, but the size of soil basal respiration rate and microbial metabolic quotients were positively influenced by the increasing heavy metal pollution levels. Microbial community structure also changed with increasing contamination, as indicated by Biolog data and principal component analysis of Biolog community metabolic profiles. Soil microbial metabolic profiles (AWCD) values, community richness and diversity index in mine-soils decreased remarkably as compared...
|
PDF Full Text Request