Immunochemical Researches Of IAA And ABP1 In The Floral Formation And Fruit Development Of Strawberry (Fragaria Ananassa. Duch.)

The strawberries {Fragaria ananasssa. Duch.) were used as the material in our researches. The immunohistochemical localization of indole-3-acetic acid (IAA) and auxin binding protein 1 (ABP1) were carried out by using an anti-indole-3-acetic acid (IAA) monoclonal antibody and an anti-ABP1 polyclonal antibody in the shoot apexes of strawberry during floral formation, the receptacles and the achenes in the developing strawberry fruits for the first time. The distribution of endogenous IAA, ABP1 and theirs dynamic variations were established. NPA was used to treat the shoot apexes of strawberry during the floral induction. Subcellular localization of IAA and ABP1 in the SAM, the floral organ primordia of the shoot apexes and in the vascular tissues of the receptacles were studied with immuno-gold electron microscopic technique. The results showed as follow:IAA and ABP1 were centralized to shoot apical meristem (SAM), the key place of floral transition, during the floral induction, gradually. IAA and ABP1 presented in the shoot apexes of strawberry all through the process of floral formation. The distributions of IAA and ABP1 were similarly during these processes. As the proceeding of the floral formation, they always localized in the floral meristem and the floral organ primordia, where including the strongest differentiated cells.NPA was used to treat the shoot apexes of strawberry during the floral induction. The processes of floral differentiation were observed, and both of the quantified analysis and localization of IAA in the shoot apexes of strawberry were studied. The results showed that the treatment with NPA at that period could inhibit the addion of IAA distribution in the SAM, delay the proceeding of floral differentiation and induced the abnormal of flower development. It was thought that the IAA in shoot apex was produced in the young leaves or/and the leave primordia, and transported to the SAM and some other places. The results suggested that the certain amount of IAA in the SAM and the construction of the system of auxin polar transport and its normal activities were essential for the differentiation and the formation of floral meristem of strawberry.Form the late phase of the immature green (IG) stage to the inception (IC) stage in the fruit development of strawberry, the obviously IAA and ABP1 signal spread all over the vascular tissues of the receptacles. The IAA signal was obviously stronger in the mature green (MG) stage and the inception (IC) stage, and mainly localized in the phloem. The ABP1 signal was always very strong during this process. Further more, after the late phase of the mature stage, the ABP1 signal appeared in the xylem and gradually increased as the proceeding of strawberry fruit development, besides the signal localized in the phloem. In the achenes, the IAA signal was increased gradually from the late phase of the immature green (IG) stage to the late phase of the mature green (MG) stage, and mainly localized in the developing embryos. After the inception stage, little IAA signal could be detected in the achenes. Form the late phase of the immature green (IG) stage to the inception (IC) stage, strong ABP1 signalalways was detect in the achenes, and the signal was mainly distributed in the developing embryos and the tissues around them.The results of the subcellular localization showed that the apparent IAA and ABPl signals localized in the cells of SAM and the floral organ primordia during all the floral differentiation and formation, and the distributions of IAA and ABPl were similarly, too, during these processes. They mainly localized in the plasmalemma, endoplasmic reticulum, cell wall, nucleolus, vacuole membrane. Among them, the signal in the plasmalemma and the endoplasmic reticulum was obviously stronger. In the mature green stage and the inception stage of the developing strawberry fruits, there were strong IAA and ABPl signal in the vascular tissue of the receptacles. Their distributions were similar, too. They were mainly localized in the cell wa...