Studies On The Dynamic Changes Of Gut-brain Peptides And Inflammation Factors In Scacculus Rotundus Of Rabbit Stimulated By Antigen

Using immuno-histochemical, tissue cultural and immuno-electron microscopic methods, based on nerve-endocrine-immune net, the dynamic changes of 4 brain-gut peptides and 6 inflammation factors of excised saccalus rotunds of rabbit stimulated by germs, LPS, exotoxin were observed. The results showed:1. After stimulated by Staphylococcus aureus , Escherichia xoli O₇8, Pasturella multocida,the content of gastrin in follicle increased significantly. This showed that the DNES cells in follicle released amount of gastrin. While stimulated by Salmonella, the results were opposite to the former. Maybe the immune inhibition of Salmonella made immune cells inhibited and through nerve-immune-endocrine system, the gastrin and other signal messages released by DNES cells were affected. The changes of VEGF decreased dramatically in follicle.2. Stimulated by Staphylococcus aureus , Escherichia .coli O₇8, LPS and exotoxin, the contents of NSE in excised SR changed most in epithelium. While stimulated by Pasturella multocida and Escherichia .coli O₇8, the content of NSE changed not so dramatically. This showed NSE most released from cells when damaged by some antigens.3. After stimulated by germs and LPS, the contents of NF in SR had no significant difference between the experiment and control group. But after stimulated by exotoxin, the content of NF in dome decreased significantly. Reckoned that NF might be regulated by nerve-immune-endocrine system..4.After stimulated by germs, the changes of contents of ecNOS were not so dramatically. While after stimulated by LPS and exotoxin, those in follicle decreased significantly.5.After stimulated by Staphylococcus aureus, Pasturella multocida ,LPS and exotoxin, the contents of CD54 had no significant difference between the experiment and control group. After stimulated by Escherichia .coli O₇8 and Pasturella multocida, the contents of CD54 decreased greatly in part of SR. This suggested that when these two germs invaded intestines, first through, the content of CD54 decreased for invasion of germs.6.After stimulated by Staphylococcus aureus, Pasturella multocida and Escherichia .coli O₇8, the contents of E-cadherin had no significant difference between the experiment and control group. While stimulated by Salmonella, the content of E-cad in epithelium decreased significantly. This showed that when Salmonella invaded intestine, the content of E-cad also decreased. After stimulated by LPS and extoxin, the contents of E-cad in dome decreased dramatically. This suggested that toxin could not change the content of E-cad but change those in dome of SR, where there were amount of immune cells and DNES cells.Infected with RHDV, the contents of SP in SR increased significantly, while the contents of actin and CD54 decreased. Ultrastructurally, the structures of SR were changed and the cells were damaged greatly. The result of immuno-electron cytochemistry showed the number of positive reaction cells of gastrin increased significantly. This suggested that SP and gastrin may interplaywith the inflammation caused by virus.All the results showed that when excised SR stimulated by various antigens, these 6 inflammation factors and 4 gut-brain peptides all increased, while SP, gastrin and NSE changed more dramatically. And those gut-brain peptides changed earlier than those inflammation factors. This suggested that these three gut-brain peptides might have a leading function in local mucosal immunity and it deduced that gut-brain peptides might have some regulative function. These results proved furtherly that SR had some self- regulative functions.