| Medicago saliva L. ( Alfalfa) is the most important forage grass in China. Nowadays, plant area of alfalfa is about 2 million hm2. One of the main problems in the production of alfalfa is the loss of decomposition because of high moisture during harvest and storage. It is estimated that the loss is about 20% of the total alfalfa output, equal to 2 million t and 2 hundred million Yuan. Silage is the idea way to resolve this problem. Ensiling has many advantages such as: good palatability, high digestibility, long storage, easy to production and mechanical operation. Alfalfa has high level of protein, high buffering capacity and low water-soluble carbohydrate content, which often leading to failure of alfalfa silage. In order to keep the alfalfa silage success, people try to add silage addition all the times to improve the alfalfa silage quality. Researches of late years testify that the technology of silage inoculants bacteria is the important way to solve the problem of alfalfa' wet decomposition.In this study, screen of alfalfa' silage inoculants bacteria, biological characteristics of silage inoculants bacteria, certification and evaluation of alfalfa silages inoculated silage inoculants bacteria and FGJ et al, were carried on. The aim of this study is to provide some valuable principles and technology bases, which can be used to resolve of alfalfa silage problem and development of high-quality and high efficiency silage inoculants bacteria.The results of this study were summarized as follows:1 In the alfalfa silages, inoculation of homofermentative lactic acid bacteria (LAB), heterofermentative LAB and combination of them could improve the numbers of LAB, impair harmful microorganism, reduce pH, NH3/TN and content of butyric acid, improve the quality of alfalfa silages. The treatments of L. buchneri+L. plantarum and L. plantarum- inoculated silages that included homofermentative LAB, had better ensile effects than others. The L. buchneri+L. plantarum and L. buchneri - inoculated silages that included heterofermentative LAB, could improve aerobic stability of the alfalfa silages. The L. buchneri + L. plantarum - inoculated silages that included homofermentative LAB and heterofermentative LAB, had best ensile effects and higher aerobic stability, and displayed positive combined effects of two fermentative LAB.2 In this study, three groups of alfalfa's silage inoculants bacteria- JD6, MMD3, X2Q2 were obtained by screening. The pH value of fermented MRS liquid nutrient medium inoculated JD6. MMD3, X2Q2 was stable during a continuous restricted cultivation, and decreased to under 3.8 after inoculated 48h. Among the metabolic products, the content of acetic acid and L (+) lactic acid was high. The component bacteria of JD6, MMD3, X2Q2 were stable during alfalfa silage. Lactobacilli (Lactobacillus plantarum or Lactobacillus pentosus) were the primary bacteria. And there were other bacteria in the three groups of alfalfa's silage inoculants bacteria.3 The three groups of alfalfa's silage inoculants bacteria could grow and breed in MRS liquidnutrient medium which added NaCl 0%—6%, or initial pH was 4 to 8, or the culture temperature range in 15℃—45℃. They could adapt a wider culture circumstance. The suitable culture temperature was 35℃. X3Q2 could adapt wider range of culture temperature, and get better silages in extreme temperature than the other.4 The silage inoculants bacteria screened in this study and silages microbial production had the same effects of alfalfa silages, and the aerobic stability of silage inoculants bacteria screened in this study were higher than silages microbial production. NDF and ADF content of alfalfa silages were unaffected by the three groups of alfalfa's silage inoculants bacteria and silages microbial production. The three groups of alfalfa's silage inoculants bacteria and silages microbial production did not affect in situ rumen DM and NDF degradability of the alfalfa silages.5 In the experiment of three different water content (70%, 50%, 30%) o...
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