Studies On Cytological Features, Biochemical Basis And Molecular Marker Of Thermo-sensitive Male Sterility In Cotton

Cotton is an important fiber crop. There is about 1,000,000 hectare cotton in China. China is the biggest country of hybrid cotton production in the world. Male sterility is very important in the utilization of hybrid heterosis in cotton. An individual plant with feature of thermo-sensitive male sterility was found in 1993 by cotton research institute of Hunan agricultural university, "Temian s-1" , the steady thermo-sensitive male sterility line was gotten by continuous 8 years' backcrossing until 2001, "D7" was used as backcrossing parent. However, as a new type of male sterilities in cotton, the cytological mechanism, physiological mechanism, biochemical mechanism and related molecular base of the thermo-sensitive male sterility has not been well explored. To know more about abortion mechanism and speed up utilization, the deep and systemic studies on morphological, cytological, physiological, biochemical and molecular studies were included in the paper, the thermo-sensitive male sterility and its near isogenic lines used as materials. Morphological markers linked to the thermo-sensitive male sterility were found by morphological observation; Stage and features of anther abortion were conformed by cytological research. The mechanism of anther abortion was clarified by physiological and biochemical studies; One RAPD marker and another SSR marker linked to the gene of thermo-sensitive were found by molecular markers choosing. The main results are as follows:Firstly, the floral apparatus of "Temian s-1" is smaller than that of "D7" . There is prominent difference between indexes such as fresh weight, length of petal, width of petal, area of petal, length of stamen, length of sigma, length of bract, width of bract and area of bract of...