Studies On Dynamic Distribution, Mucosal Immunity And Influence For Intestinal Microbial Community Of Ducks Infected With Duck Plague Virus Virulent Strain

Duck plague(DP), also named duck viral enteritis (DVE),is an acute, septicaemic and contagious disease of the anseriformes including ducks, geese and swans caused by duck plague virus infection. Mucosal immunity is an immune mechanism located on mucous membrane surfaces of gastrointestinal, respiratory and urogenital tracts. The mucosal immune system may, to some extent, be independent from the systemic immune system. It was tolerant to majority harmless matters or nutrient substances and can be induced immune responses to some pathogens. MIS is an important line of defense against invasive pathogens on the mucosal membranes. The reaction of local mucosal immunity and molecular characterization of intestinal microbial community of ducks infected by different routes with duck plague virus virulent strain had been detected, and the contents are summarized as follws:1. Duck immunoglobulin A (IgA) was isolated from bile by ammonium sulfate precipitation and different concentration of polyethylene glycol (PEG-6000) extraction and purified by Macro-Prep S after above steps. Purity and antibody activity of IgA were analysed by SDS-PAGE and ELISA. The results indicated that the highest output and purity of IgA was obtained by the PEG precipitation with 15% PEG. IgA isolated by 22% PEG and precipitated by ammonium sulfate was further purified by affinity...