The Identification Of Proteins Relating To Embryonic Development And Their CDNA Cloning In Seedless Litchi.

Litchi chinensis Sonn is an important agricultural product of subtropical Southern China. Hainan seedless Litchi is an excellent variety with a high seedless rate of 95%. Unfortunately, the seedless rate is unstable and dependent on the temperature during flowering stage. The unstable seedless rate seriously impacts the seedless Litchi's planting area and its commercial value. Although there are many morphological biochemical studied during embryo developing of litchi, there are few studies on the proteins and proteomatic relating to the embryonic development of litchi.The purpose of this research is to isolate the proteins of seedless Litchi at the different embryo development stages by using the two-dimensional polyacrylamide gel electrophoresis (2DE) in order to isolate the differential proteins between normal embryo and aborted embryo. The next step is to identify these proteins by using the MALDI-TOF MS search homologous protein and obtain their sequence through the protein data bank.The major results of the study are as follows:1. From 2DE electrophoresis maps, we compared the global protein patterns between normal and aborted embryo of litchi at the 20 DAA (day after anthesis) through computer image analysis. In total, 129 and 130 protein spots were obtained in the normal and aborted embryo maps, respectively. Of these, 24 spots were not significantly different, while 14 spots increased and 21 spots decreased in quantity. Furthermore, some spots were missing and some new spots emerged in aborted embryo compared with the normal embryo. The differential proteins between normal and aborted embryo of litchi are useful for regulating and controlling embryo abortion.2. We compared the global protein patterns between normal and aborted embryo of litchi at the 40 DAA. The result shows, the rate of the differential proteins were increased compared with the 20 DAA. 388 and 482 protein spots were obtained in normal and aborted embryo map, respectively, of which 60 spots were not significantly different, while 46 spots increased and 54 spots decreased in quantity. Furthermore, some spots...