Hereditary Analysis Of Peculiar Wheat Germplasm Derived From Wheat-Agropyron Cristatum

Cytological, in situ hybridization, gliadin, molecular markers and morphological Stat. were employed to detect and analyze two kinds of peculiar wheat germplasm derived from wheat (Triticum aestivum L.) - Agropyron cristatum (2n=28, genomes PPPP). Major result is as follows:1. To detect exogenous hereditary property of the derive descendants from wheat- A. cristatum A large-spike with more floret wheat germplasm (4844) and earing inhibition plant lines were obtained by screening the descendants from hybridizing between common wheat (T. aestivum L.) and Agropyron cristatum (accession Z559). The results of genomic in situ hybridization (GISH) detect, a disomic alien chromosome addition line 4844-12 and two chromosome substitution lines 4844-2 and 4844-8, in 15 progeny lines of 4844. GISH result still shows, these earing inhibition plant lines belonged to the translation lines between two couple wheat chromosomes and A. cristatum chromosomes. Total six translational markers were detected by using SSR analysis in them.2. The hereditary analysis of the supper spike wheat- A. cristatum addition line and substitution lines In order to determine the genetic control of multiple florets and kernels per spike in this line, chromosome addition and substitution lines that were derived from line 4844 were characterized by means of in situ hybridization, microsatellite (SSR), and gliadin analyses. Genomic in situ hybridization analysis with biotinylated P genomic DNA of A. cristatum as a probe demonstrated that the increased number of florets and grains in a spike was associated with the A. cristatum chromosome introgressed. Fluorescence in situ hybridization, using a repetitive sequence, pAs1 derived from Aegilops squarrosa L., indicated the replacement of chromosome 6D of wheat in the wheat-A. cristatum chromosome substitution lines. This was confirmed by microsatellite analyses with wheat SSR markers specific for chromosome 6D, suggesting that the A. cristatum chromosome was homoeologous to group 6 and was therefore designated 6P. This verdict was further confirmed by amplification using EST-SSR markers and gliadin analysis. The increased number of florets and kernels within a spike of the wheat-A. cristatum hybrids was thus controlled by gene(s) located on A. cristatum chromosome 6P. At the...