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Identification And Genetic Analysis Of The Resistance Of Wheat-Psathyrostachys Husashanica Off Spring To Take-All

Posted on:2009-05-15Degree:MasterType:Thesis
Country:ChinaCandidate:F Q WeiFull Text:PDF
GTID:2143360245450890Subject:Seed project
Abstract/Summary:PDF Full Text Request
The relative species of wheat has the quite rich genetic diversity and excellent genes which can improve wheat, strengthening the cross between wheat and the relative species, identification and utilization research, will promote the production and breeding of wheat. This research through the cytological analysis, in situ hybridization, molecular marker and Statistical Analysis on Morphology, some resistance Take-all lines of the stable derived lines of wheat–Psathyrostachys huashanica(2n=14,NN).were identificated Systematically and analysed. The main result is as follows:1.Resistance Take-all indentification of wheat-Psathyrostachys huashanica progeny and cytology analysis and GISH analysis of resistance lines By GISH detection,we obtained 3 resistance lines from 8 stable lines. H9017 is addition line and H922 is substitution line,but hybridization signal was not detected in H924, we can conjecture it is a translocation line with small fragments or introgression line into which anti-Take-all gene was transferred.2. The hereditary analysis of anti-Take-all of F2 group of H924 By IECM algorithm estimation result for the F2 group resistant and susceptible character of 96(15)×H924 show, Inheritance of Resistance is from two pairs of main gene and many gene control, main genic hereditary model is B-1,F2 group main genic hereditary rate is 96.7% and 94.6% respectivly.3. The chromosome location of anti-Take-all gene of H924 Via wheat SSR molecular mark technology to amplify genome of parents by 296 pairs of primers randomly distributed along the linkage map in (common)wheat we got 102 pairs of primer which can amplify polymorphic bands,5 pairs of these primers was polymorphic betweenhe resistant and susceptible bulk, primer which can amplify polymorphic bands appears most frequently in 5D,there were 3 pairs of primers,there were 2 pairs of primers in 6D .
Keywords/Search Tags:Wheat- Psathyrostachys huashanica derive descendants, Resistance to tale-all, Genetic analysis, Chromosome location
PDF Full Text Request
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