Identification, Mapping, Characteration Of Expression, SNPs Detection And Association Analysis Of The Twevel Genes With Part Traits In Beef Cattle

Recently, the animal nutrition study was focus on the individual as a whole, that means treat the animal with the certain nutriment then test its effect on metabolism, energy balance and absorption efficiency at the body level. On the other side, molecular basis for these mechanisms were still obscure. All of the biological and biochemical alterations of the animal, such as development, differentiation, metabolism, immunity and disease, could be boil down to the gene regulation changes. In one word, most tangible changes could trace back to the nucleotide. So, a combined research of animal nutrition and molecular biology could shed great light on the mechanism of how every kind of material effects in body.The digestive system of cattle is a complex. Considering the complicated structure of alimentary canal of cattle, especially the rumen, varies nutriments couldn't be fully transformed and absorbed there to achieve high efficiency. On the other side, the inside mechanism of energy circulation and utilization in cattle appeared more complicated. So, great challenge was ahead waiting for the ruminant nutritionist. In this study, we employ the methods of molecular biology and bioinformatics to isolate several genes which play an important role in ruminant digestion. We have cloned sequences of 12 genes and assigned 10 to bovine chromosomes, and more related information was also included.1. The mRNA sequence for the human ortholog gene was obtained from NCBI, and was employed to search for bovine expressed sequence tags (ESTs) in the EST-others database through standard BLAST algorithm. The bovine ESTs that have more than 80% similarity with the corresponding human mRNA were candidates, assembled into a contig and used to design the gene-specific primers. We obtained part sequences of twelve genes in the genome of sanhe cattle. These genes are as follows: Cholcystokinin (CCK); Glutamate decarboxylase (GAD1 and GAD2); Gastric inhibitory polypeptide (GIP); Galanin (GAL); Fructose-1,6-bisphosphatase (FBP1); Phosphatase (PSPH); Carboxypeptidase (CPB1); Proteasome 26S subunit, ATPase, 1 (PSMC1); Proteasome 26S subunit, ATPase, 2 (PSMC2); Proteasome 26S subunit, ATPase, 4 (PSMC4); Proteasome 26S subunit, non-ATPase1 (PSMD1), the accession number are DQ111991; DQ111992; DQ111993; DQ111994; DQ111995; DQ118401; DQ118402; DQ120511; DQ120512; DQ120513; DQ139315; DQ139316; DQ139317; DQ146944; DQ160296 and...