The Study On Mechanism Of Prolificacy In Hu Sheep

The trait of prolificacy of sheep was an important approach increasing more economic benefit. In recent years, a major gene (FecB) controlling sheep prolificacy in Booroola in Australia was fond, and its genetic traits, genetic effects and other characters were widely studied. During several years after this find, FecB gene was widely studied as a candidate gene in many countries in different sheep breeds. The FecB gene was found in Hu and little Han in our country, which were two famous prolific sheep breeds in the world. To date, about the biological effects, bio-chemical characters and effects on hormone secretion of FecB gene in different breeds or different animals in the same population had no clear reports. To increase litter size and decrease mortality rate of early embryos, the study on uterus inner conditions from histology, physiology and reproduction hormone level must have been processed. It may be some rules were found about prolific sheep breeds and to provide a method for molecular marker assistant breeding.Many technologies, such as RIA, nucleic analysis, gene marker assistance and so on were used to in this experiment study prolific trait of Hu, and using China Merino Xinjiang reclaimation sheep as control, to enrich the theory of sheep reproduction mechanism, this study had important significance for increasing litter size and decreasing embryos mortality and increasing economic benefits for sheep breeding. The main contents presented as follows:1. Study of the level of hormone in early pregnancy of sheepTo study the prolific mechanism of Hu Sheep in Jiangsu province, many techniques such as RIA, nucleotide analysis, gene marker analysis and so on were used in this experiment to study prolific trait of Hu Sheep, and using China Merino Xinjiang reclaimation sheep as control. Many effect factors on sheep reproduction were studied between Hu and control group, such as oestrus cycles, level of FSH, Leptin, PGF_2α, progesterone and estradiol in blood of outer blood vessel and content of DNA and RNA or...