Analysis Of6KRT Gene Genetic Variation And Its Relationship With Wool Traits In Chinese Merino (Xinjiang Type) Sheep

The first application of DNA sequence analysis,PCR-SSCP techniques and analyze the geneticvariations of candidate gene and their association with wool.traits in418Chinese Merino(Xinjiang type)sheep.The Keratin family of six candidate genes17loci polymorphism variation，included KRT27gene(exon3,8and intron2,5), KRT31gene (intron2), KRT36gene (exon1,2,3),KRT38gene (exon3,intron2,7), KRT81gene(exon1,2and intron1,3), KRT85gene (exon2and intron2).Using leasts quares varianceanalysis (SAS8.1), analysis and wool traits (wool staple length, wool bending points,wool fineness score,number of bending,evalution weight,body size,identification of when weight,fleece weight, average fiberdiameter wool trians,coefficient of variation wool fiber).Improvement Chinese Merino (Xinjiang type)wool trains,and gene pyramiding effect on the Chinese Merino wool traits.as well as of candidate genesassociated with wool,provide a scientific basis.This test analysis results in the following:1．KRT31-Q1gene had CC,CD tow genotypes in Chinese Merino (Xinjiang type) sheep.The C alleledominant, accounting for66%of the groups; Missense mutation in the51bp insertion；KRT31gene wasat Hardy-Weinberg equilibrium (P>0.05) in the Chinese Merino (Xinjiang type),and moderate polymorphiclocus.Wool fiber diameter significantly different (P<0.05),therefore, the KRT31gene could be used as awool fiber diameter of a DNA molecule mark.2．KRT36-R3gene had EE、EF、FF three genotype in Chinese Merino (Xinjiang type) sheep.The F alleledominant, accounting for66%of the groups; A locus deletion, two point mutations, respectively62A/-,63T/A,83A/T.caused by missense mutations in the amino acid sequence was found by analysis ofthe amino acids encoded;KRT36gene was at Hardy-Weinberg equilibrium (P>0.05) in the Chinese Merino(Xinjiang type), and moderate polymorphic locus; fineness of wool, wool fiber diameter was significantlydifferent (P<0.05);the KRT36gene could be used as fineness of wool fiber diameter of the selected DNAmolecule mark.3．KRT38-S2, KRT38-S3gene had MM、MN、NN three genotype in Chinese Merino sheep (Xinjiangtype) genotype. S2non-coding region appear to85bpG/A,95bpC/T;S3coding region, they are showedrespectively93T/C,97A/C and111G/C,112A/T,126G/T mutation sites.Caused Ile�'Thr,Glu�'Asp,Gly�'Ala,Ala�'Ser.Caused by mutations in the translated amino acid sequence, which may have an impacton the functional properties; Chinese Merino (Xinjiang type) groups in this locus is in Hardy-Weinbergequilibrium, are moderate polymorphism;the KRT38-S2gene Wool not significant (P>0.05), KRT38-S3genes and the number of wool bend the differences between significant (P<0.05);the KRT38gene could beused of wool bending DNA molecule marker.4．KRT85-W1genes had PP, PQ, QQ three genotype in Chinese Merino sheep (Xinjiang type) genotype,W2coding region appears49A/A,210G/A,354G/A mutation sites.respectively, caused byThr�'Thr,Trp�'-,Gln�'Gln; KRT85-W2gene was at Hardy-Weinberg equilibrium (P>0.05) in the ChineseMerino sheep(Xinjiang type), and moderate polymorphic locus; wool bending, the physical size of thescore, the diameter of the wool wool significant difference (P<0.05); the KRT85gene could be used ofwool bent, wool fiber diameter selected DNA molecule marker.5．Chinese Merino sheep(Xinjiang type) group, the excellent combined genotypes26group, thecombined genotype effect is obvious FF-MM-PP is the dominant genotype;EE-MM-PP,EE-NN-PQ,EE-NN-QQ,FF-NN-QQ,EE-MN-PQ,EE-MN-QQ,6combined genotype combination,showing theperformance best hair candidate gene combination.Provide some reference for the further development ofChinese Merino (Xinjiang type) wool multi-gene pyramiding breeding.