Investigation Of The Germplasm Resources And Biological Characteristics Of Seeds Of Radix Platycodonis

The results observed from the characters of flower,capsule,seed and blade of the germplasm resources of Radix Platyeodonis indicate that: The flower, capsule, seed, blade property of Radix Platycodonis have a lot of variation types, property these until different source plant between the source have obvious difference, and these properties on the same plant are all the same, can regard as genetic variety, offspring of breeding can as genetic mark, can also regard as, distinguish different kinds of the germplasm resources.The results observed from 8 agronomical characters that highness of trunk,wide of stem,leaf numbers of aulis,fresh weight of aerial part,length of main root,numbers of lateral root,wide of root,weight of single root of 50 germplasm resources of Radix Platycodonis indicate that: the variation spectrum of these agronomical characters are relatively greater,and it will be usefull for new variery breeding programs;the results of correlation analysis, multiple regression analysis and path analysis indicate that: the affect of wide of root and wide of stem to weight of single root is relatively great,and it will be used as target to single-tree selection.The results determine the total saponin content of 25 germplasm resources of Radix Platycodonis indicate that: the total saponin content were differ from each other among the germplasm resources,the high content of saponin resouces sach as.日本美里紫will be used in breeding programs directly or used as basic resources of officinal variety breeding programs.The results of RAPD analysis of germplasm resources of Radix Platycodonis indicate that: although the primers and samples were differ in twice examination,but the results were similar.in twice examination,all the samples were grouped to 7 and 8 groups,individually, and reveled that all the samples grouped in geographically.It will be used as referrences of distinguish the germplasm resouces of Radix Platycodonis.From the OPG02 primer, obtained an about 580bp DNA fragment that observed all of the Platycodon grandiflorum(Jacq.) A.DC samples but couldn't observed from Plandicodon grandiflorum(Jacq.)A.DC, var.album Hort..It is indicate that the fragments sfecific to Platycodon grandiflorum(Jacq.) A.DC and will be used as molecular markers to distinguish the two kinds of Plandicodon grandiflorum.The result that analysis of the reason of low self-seeding and study of the solution of Radix Platycodonis under the natural state indicate that: the stigma can accept pollens from own or other flower's, the reason of low self-seeding is mainly due to differences of the female and stamen development period in the same pieces of flower, the problem of low self-seeding rate can be solved by preserving the pollens artificially and by artificial pollination.The relations analysis result of the seed mature tendency of Radix Platycodonis with the main climatic factor indicate that: for complementery mature of the seed,it is need 55 dayes from flowering; and 31 to 35 dayes from flowering were the fast stages of increesing thousand kernel weight and mature rate of seed. in nature, if satisfy the developmental conditions, the courses of maturation were mainly controled by own developmental mechanism, the meteorological condition can not remarkably affect mature advancement of the seed, but the seed mature period temperature affects the seed thousand kernel in a certain extent.The analysis results that the seed maturity influences to the germination percentage and the rate of seedlings emergence indicated that, from the seed maturity approximately is 60% start, seed's final germination percentage can achieve above 80%, but germinates peak and seed maturity related, maturely goes past the high seed to germinate the peak more ahead of time, the seed rate of seedlings emergence is also higher vice versa.The results that processing different maturity seed's with 0.3% potassium permanganate and the 10mg/L gibberelin indicate that: 0.3% potassium permanganate and the 10mg/L gibberelin process to the different maturity seed effect different, to the maturity good, germinates needs the time short seed, has the function which reduces or postpones germinates, to the low mature rate, germinates needs the time long seed, 0.3% potassium permanganate and the 10mg/L gibberelin promotion seed germinate the function is very obvious.