Research On Genetic Relationship Of Lilium Species And Conservation Genetics Of L. Regale

China is nature distributional center of Lilium species, Researches on wild resources are an important base to breed new cultivars.(1)The distribution, ecology environment of 19 Lilium species and 2 unknown types in most of Southward of China and northeast of China were investigated and collected. L. brownii var. viridulum, L. lancifolium, L . tsingtauense andL . speciosum var. gloriosoides, L. dauricum, L. concolor, L. distichum,L. pumilum,L. brownii var. viridulum,L. brownii,L. lancifolium,L. leucanthum ,L. brownii, L. brownii var. viridulum,L. regale, L. sargentiae, L. sulphurreum, L. henryi, L. davidii, L. rosthornii, L. duchartrei, L. lophophorum and L. sp.1, L. sp.2 were investigated and collected.(2)Genetic relationship was analyzed based on the data of pollen morphology, ISSR molecular marker, ITS sequences of nrDNA and trnL-F sequences of cpDNA from each species. The result show that there were closely relationship among L. rosthornii, L. lancifolium, L. sp.2, L. davidii, L. concolor;among L. brownii var. viridulum, L. brownii, L. sp.1;betweenL. Tsingtauense and L. distichum, and among L. dauricum, L. sulphureum, L. leucanthum, L. regale, L. sargentiae.(3) The genetic diversity and spatial autocorrelation of genetic structure of L. regale was analyzed base on ISSR molecular marker. At the species level, the proportion of polymorphic loci was 97.7%, the effective number of allele (Ne) was 1.994 4, Shannon diversity index (I) was 0.333 9. At the population level, the average expected heterozygosis was 0.664. Shannon diversity index (I) was 0.272 0. They showed that genetic diversity of L. regale was high. The Gst of L. regale was 0.161 9,(Hsp-Hpop)/Hsp was 0.153 7,the result by AMOVA analysis indicated that the variation within population account 83.8% and variation among populations accounted for 11.849%, and the gene flow was 2.588. They demonstrated that the relationship of populations was closer. Gene differentiation was acute within population. The spatial autocorrelation of genetic structure of 4 populations were analyzed. The Moran's I correlogram revealed no significant spatial structure in the 4 populations. It indicated that genetic variations of the most polymorphic loci in these populations were randomly distributed.However, there were gaps at the distance of 3～4m, 5～6 and 8～10 m , and there were intrusion at a little polymorphic loci.