Research On Follicular Dynamics Of Goat And It's Application On Goat

Transrectal utrasonography of ovaries were performed each day in prolific Lubei White does(n=7) during 3 oestrous cycle in two different breeding seasons in Autumn and in Spring, and for 32 days in the middle of anoetrous season in Summer to record the number and size of all follicles≥3.0mm in diameter.And Transrectal utrasonography of ovaries were performed each day in non-prolific Yimeng Black does(n=6) and prolific Lubei White does(n=7) during 3 oestrous cycle in the breeding seasons in Autumn to record the number and size of all follicles≥3.0mm in diameter. Follicular data were then classified by the number of follicular waves to determine the folicular diameter range of follicular diviation.The observed number of growing identified 4-mm follicles per day differed (P<0.05) from randomness, indicating that follicles, on the average, emerged in groups (waves). Averaged over all interovulatory intervals, the number of 3-mm follicles on each day that later reached≥5 mm followed a pattern of significant peaks on Days 0 (ovulation), 4-5,10 and14-16. A follicular wave was defined by consecutive days of entry of follicles≥5 mm into the wave, and the day of emergence was defined as the first day that the≥6 mm follicles were 3 mm. Follicular number decreased rptly in the diameter range from 3.8mm to 4.5mm, and the folliclle quantity decreased unsignificantly in the the diameter range >4.5mm. 4 waves were observed in 12(60%) interovulatory intervals in Autumn, while 3 waves in 6(60%) interovulatory intervals in autumn in Spring, and no difference were found in the interovulatory intervals of the Lubei White does between Autumn and Spring. The follicular wave were also observed in the anoestrous season in Summer, the interwave intervals is 4.4 in first half month during the anoestrous season in Summer,while in another half monthe it proloned significently. No difference about recruitment phase and follicular numbers emerged during the period of ovulatory wave recruitment phase in ovulatory wave were found between the two breeds of goat differing in prolificacy. However the dominant follicular number and ovulatory rate were higher significently in prolific Lubei White does. And the penultimate wave found contained ovulatory follicles in 11.1% of non-prolific Yimeng Black does but in 35% of prolific Lubei White does.80mIU or 160 IU FSH per day per goat was intravenous injected to the two experiment group of non-prolific Yimeng Black does next day when follicle >4.0mm diameter was observed during the ovulatory wave in Autumn, for two days,while the control received vector only. Blood samples collected twice a day from the day follicle reaching >4.0mm diameter the the day on heat, and were analysed by radioimmunoassay for contentrations of FSH. The does in vector were allowed to be mated by fertile bucks during estrus. Ultrasonographic examations were performed transrectally to detected ovulatory follicle numbers and pregnancy on 28 days post-mating. Serum FSH concentrations were keep invariable or little increase in the experiment groups while the Serum FSH concentrations decreased in the group. Two or more follicles reaching >5.5mm in diameter and ovulation were obtained in the experiment groups, and 1.67±0.57 fetus were observed on 28 days post-mating. 151 Boer goats were superovulated to examine the factors affecting superovulation and embryo transfer (MOET). An optimal regime for superovulation treatment was identified as a 4-day treatment with decreasing dosages of 6-7 mg Chinese FSH or 240 mg Canadian FSH. The 4-day treatment with decreasing dosages of 6-7 mg Chinese FSH was, therefore, adopted to study effects of the age of does, season and repeated treatments on superovulation and embryo transfer. The best season for superovulation and embryo transfer and pregnancy was Autumn, and the best age range was 12-35 months old. No significant difference among the animals were found in the number of ovulations and the rate of transferable embryos between the first and the second superovulation. However, these parameters declined significantly for the third superovulation. No marked effects of the number of ovulations on the proportion of transferable embryos were noted. The parturition rate of the recipients receiving single embryos was not different significantly from those receiving two embryos, and the kidding rate calculated from embryos transferred did not differ significantly between recipients receiving one and two embryos.