Study On Exploitation And Application On Tinospora Sagittata (Oliv.) Gagnep

Objective:The project is a system research on Tinospora capillipes Gagnep.crude drugs,which include studying on quality standard,extraction process,chemical components, pharmacological actions et al..According to the system research on Tinospora capillipes Gagnep,the project will clarify the relations between components and pharmacological actions,establish the optimum extraction process for Tinospora capillipes Gagnep,institute preparation and quality control of Jinshan yansukang spray.Method:1.Study on the chemical constituentsSeparate extract of Ethyl acetate of Tinospora sagittata(Oliv.) Gagnep by chrotamography method.identify the extract of Ethyl acetate by ¹³C-NMR.EI-MS.2.Study on quality standard detecte ingredients of Tinospora capillipes Gagnep with the methods of Pharmacopoeia 2005 edition adopted in Appendix,such as water,ash ect. Establish TCL identificaqtion for inospora capillipes Gagnep,Establish the method for the content determination of jatrorrhizine in inospora capillipes Gagnep by TLC scanning and the content of Palmatine hydrochloride and jatrorrhizine hydrochloride by HPLC.detect 10 Tinospora capillipes Gagnep by developed method.3.Study on the extraction process Research the relation between Pharmacodynamics site and Pharmacological effect With Pharmacological Tracer,search high efficiency Pharmacodynamics site,Compare different extraction methods of Tinospora capillipes Gagnep,study on the heat reflux extraction method for Tinospora capillipes Gagnep by Orthogonal test,and semi-bionic extraction method by homogeneous design method.4.Study on the pharmacology functionStudy on anti-inflammatory effect of Tinospora capillipes Gagnep by significant inhibitory effect on the xylene induced ear edema,by the effect on the acetic acid induced permeability Increased of Peritoneal Capillary,by the effect on the Histamine induced permeability Increased of Skin Capillary,by the effect on the Egg white induced foot edema.Study on Anti- Gastric ulcer of Tinospora capillipes Gagnep by the effect of disease caused by the fail in emotion in Tinospora capillipes Gagnep,by the effect of xiaoyantong induced peptic antiulcer in rats,by the effect of Aspirin induced peptic antiulcer in rats,by the effect of pylori Ligation induced peptic antiulcer in rats,by the effect of Ulcer healing induced by acetic acid.Study on the effect of blood glucose depressing induced by Alloxan in rats,compare the effect of blood glucose depressing between Tinospora capillipes Gagnep and Tinospora sagittata(Oliv.) Gagnep.Search the rule of vivo metabolism of Tinospora eapillipes Gagnep in animals by blood fingerprint and Antibacterial test.5.The preparation technology of SprayTo optimize formulation and preparation technology at the basis of Total alkaloids of Tinospora capillipes Gagnep,and made a study on the Quality control Standard for the optimized formulation.Results:1 Herbalism study on Radix Tinosporae has been accomplished.According to the result of the herbal works,the real origine of the sampleshas has been identified as Tinospora sagittata(Oliv.) Gagnep.2.A elementary syudy on Chemical constituent has been accomplished.The study isolated pure compounds by using repeated column chromatography,while the structure of new compounds were determined by detailed spectral analysis.Four compounds were isolated from the decoction of the roots of Tinospora sagittata(Oiiv.) Gagnep,which are Columbin,Palmatine,Isocolumbin and Tinoside.3 A system quality standard has been established.The primary contents include ingredients water,ash,acid insoluble ash,extract,identify and assay method,and other related quality standards.4 Compared Palmatine in Tinospora sagittata(Oliv.)Gagnep from different Habitats,the sample from Wudang mountain in Hubei province was the best.It can be cultivated as the best seed quality resourse for Good Agricultural Practice.5 Study on the optimum extraction process of Tinospora sagittata(Oliv.) Gagnep.crude drugs by Orthogonal Design.In order to optimize the conditions of the extraction technique for Tinospora sagittata(Oliv.) Gagnep.crude drugs,HPLC Method was used to analyze the content of atrorrhizine Hydrochloride and Palmation Hydrochioride,and the orthogonai experimental design was introduced to optimize the conditions of the extraction technology.The experiment of this part included ethanol,amount of ethanol,reflux timeand times of extraction,involved in four factors,which carried out by orthogonal design.The following Results have been obtained:The conditions of the ethanol extraction technology of extracting the constituents from Radix Tinosporae were 95%ethanol,6-fold solvent,0.5 hour of reflux and 2 times of extraction.The results show that the process is reasonable and reliable.6 Pharmacological actions study on Tinospora sagittata(Oliv.) Gagnep.The pharmacological actions of Tinospora sagittata(Oliv.) Gagnep.were preliminarily investigated in the dissertation.The experiment showed that aqueous extract of Tinospora sagittata(Oliv.) Gagnep.has significant anti-inflammatory effects against acute inflammation and immunity inflammation.It remarkedly decreased the parameters of ulcer,and possessing promotion of ulcer agglutination.It has a obviously amelioration effect against gastric stress ulcer. The results show that the effects of reducing blood sugar of Radix Tinosporae of is better than ofTinospora sagittata(Oliv.) Gagnep.7 preparation and quality control of Jinshan yansukang spray.To prepare Jinshan yansukang spray and establish its quality control methods,the preparation based on the original drug extract,dissolved,filterde,adjusted the pH value,packaged in spray containers.TLC identification and HPLC methods were used.Conclusion:After system studied on Tinospora capillipes Gagnep,The project preliminary identified the Choiceness Varieties of Tinospora sagittata(Oliv.) Gagnep,developed Quality control Standard methods of Tinospora sagittata(Oliv.) Gagnep,improved safety and effectiveness of the crude drug for Tinospora sagittata(Oliv.) Gagnep,examined Pharmacological activity of Tinospora capillipes Gagnep in Antiulcer,anti-inflammatory and blood glucose depressing,established the best process of heat reflux extraction for Tinospora sagittata(Oliv.) Gagnep,developed the process of Jinshan yansukang spray and the quality control standard for it,above all the research lay a solid foundation for resource development of Tinospora capillipes Gagnep.