Analysis Of Differential Proteomics On Longan (Dimocarpus Longan Lour.) Floral Reversion And Normal Flowering

Proteomics method was used to compare and identify the differential proteins of normal flowering and floral reversion buds in longan (Dimocarpus longan Lour.). over 1000 protein spots were detected on 2 DE gels, PDQuest was applied on gel pattern analysis, 65 differentially expressed proteins were detected. Among these proteins, 28 were up-regulated and 37 were down-regulated on floral reversion buds. 41 differential proteins were identified by MALDI-TOF-TOF/MS and protein database searching, the identification rate was 63%. Biological function analysis showed these identificated proteins related with substance and energy metabolism, transcription and translation, secondary metabolism, regulation, stress tolerance and cytoskeleton. Open reading frame(ORF) of up-regulated protein regulation factor 14-3-3(GenBank Accession No. FJ479618), cytoskeleton alpha-tubulin(No. FJ479617), transaldolase(No. FJ472991) and down-regulated anthocyanidin synthase(No. FJ479616)were cloned with RACE method. All 4 genes were expressed in E.coli, and western blotting confirmed 14-3-3 and alpha-tubulin. Semi-Quantitative RT-PCR result showed ANS and TAL each has identical regulation on transcription and translation level, whileα-tublin and 14-3-3 each has minor expression differential on transcription level. The changes in expression level of these proteins might cause flower buds developing abnormally and lead to flowering reversion in longan.The results has an important significance in revealing the molecular mechanism of flowering reversion in longan.