| Long term and excessive drinking is the base of alcoholic liver disease (ALD). Hepatic cell can be injuried by excessive drinking,.with the progression of ALD, alcoholic fatty liver, alcoholic hepatitis, alcoholic hepatic fibrosis and the telophase of ALD-alcoholic Hepatic cirrhosis occur in succession or simultaneously. In developed country, the morbidity of ALD is much higher than other liver disease. Especially in serious alcohol abused country, such as American, French, Germany, Italy and Spain, incidence rate of alcoholic liver disease occupies 50%-75% in all cirrhosis of liver. Meanwhile alcoholic cirrhosis occupies 25%-30% in Asiatic Japan. The distinct of geographical distribution, diet structure, habituation of drinking, epidemic of viral hepatitis, and race microsomal ethanol oxidizing system (MEOS), alcohol dehydrogenas, aldehyde dehydrogenase polymorphism variation, episode constituent of alcoholic liver disease are various. In two investigations of eastern and western countries, the proportion of pathologic type of ALD, fatty liver, alcoholic hepatitis, hepatic fibrosis, cirrhosis of liver, chronic hepatitis and unspecific variance, are 46%> 31% ,11%>8%,0%NO% (breach type in western countries sand in Japan, the proportionare 12%, 10%, 25%> 33%> 10%, 11%. Besides, as the proportion of the population with Hepatitis B or Hepatitis C are higher than western countries in Japan, the hepatocellular carcinoma morbidity is also higher than alcoholic hepatic cirrhosis. In present studying, pathologic change in different ALD stage has different pathogenesis. Alcoholic fatly liver is related to lipid metabolism, lipo-acid oxidation blocking, lacking of apolipoprotein, VLDL allo-excretion; alcoholic hepatitis is related to toxic effect of monocyt, insufficiency of glotathione-SH, oxygen-derived free radidicals gaining, cytokine and inflammation medium accumulating; hepatic fibrosis is related to inflammation by acetaldehyde, transforming growth factor-β(TGF-β, platelet derived growth factor (PDGF) and immunologic reaction result in activation of hepatic stellate cell and Inhibition of collagenase. With Synthesis of collagen and reduction of degradation, repeated inflammation and fibrosis result in alcoholic cirrhosis.With the developing of our social economy, production and consumption of liquor increase. Impairment to the health .especially to liver gradually shows. Several national and regional epidemiological survey has been carried out in our country since 80's, but these survey focus on alcohol addiction and about psyche , nerves . We choose the Zhejiang Changguang coal mine as the survey region in this epidemiological survey. It involves in population with different occupation. Questionnaire includes drinking capacity for liquor, drinking frequency and morbidity of alcoholic liver disease. And some laboratory examination was carried out. In the topic, the population in this survey was in sampling action. Variable number of tandem repeat (VNTR) polymorphism of nterleukin-1 receptor antagonist gene (IL-1RN) and single nucleotide polymorphisms (SNPs) of the alcohol metaboliting related enzymes were analyzed. Also we want to ques the the relationship between the genes polymorphisms and alcoholic liver disease, In order to explorating the correlation with different stage or severe degree, Assayingthe peripheral blood mononuclear cells (PBMC), the expression of TGF- P i in patientwith ALD were quantitative analysised. Thus we study the molecular biology mechanisms relating with ALD advancedly. Epidetniological survey and experiment methodsEpiderniological material and methods Survey region was Changguang coal mine, objects Were various kinds occupation population working in Changguang coal mine region, for instance worker performancing under coal mine and institution rear service people. Stratified sampling 4310 people coincidenced with survey standard. Reject unqualification object, there are 4084 datas altogether. Qualification rate was 99.05%. General information of al...
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