Effects And Molecular Mechanisms Of Anti-Schistosoma Japonicum Egg Granuloma Induced By SCP Extraction Combining Reagent

Part I Preparation of Shark Cartilage Powder (SCP) Extraction and their immunogenicity characterization Objective To investigate the immunogenicity characterization of T lymphocyte phenotypes and specific antibody responses induced by SCP extraction in normal saline (SCP1) and in guatudine hydrochloride solution (SCP2). Methods Fifty Kunming male mice were divided evenly into 5 groups (from group I to V). Mice were immunized with SCP1 at the smaller dose of 5O^g/O.2ml protein content per mouse (group I ) or at the larger one of 1OO^tg/O.2ml (group II), respectively. Group III and group IV were immunized respectively with the smaller or larger dosage of SCP2. the dosage was the same as the above^mentioned. Group V was injected with normal saline as a control group. Two weeks later after the second boosted immunization (six weeks after first immunization), mice were killed for determinations of the following indices. The percentage of splenic T lymphocyte phenotypes of mice was detected by flowcytometer. The serum specific antibody IgG and its subclass IgG1 and IgG3 levels were determined by ELISA. Results The results showed that the percentages of splenic CD or CD8 in group I ^. II III and IV were significantly higher or lower than those in group V. The ratios of CD4 / CDg^1.45--1.92 in group V.. IL.. III and IV were obviously higher than that (1.15) in group V (pmd ^kI^ctiIm Mechanisms of Anti ^shutosoe,a,apcirnosim E ilTanuloma h,duncd by SCP Extnidisui Coné¼…utm keapⁱs Abstracts (group IV) were more intensive than those by the smaller dosage of SCPI (group I) and SCP2(group III), respectively (p