Study On Separation And Purification Of Shark Cartilage Polysaccharides And Their Anti-Rheumatoid Arthritis Mechanisms

Rheumatoid arthritis(RA) is a common systemic autoimmune disease, which is characterized by chronic polyarthritis symptom. According to the RA pathogenesis studies, anti-inflammatory therapy has become one important treat principle. It is reported that glycosaminoglycans(GAGs) has anti-inflammatory effect in recent years. Shark cartilage polysaccharides(SCPs) was selected as anti-rheumatoid arthritis materials in our study.SCPs mainly composed of keratan sulfate(KS), chondroitin sulfate A(CSA), chondroitin sulfate C(CSC) and hyaluronic acid(HA), and all of them belong to the family of glycosaminoglycans(GAGs). The major disaccharides of CSA and CSC are glucuronic acid and sulfated N-acetylgalactosamine, which are connected by β1→3 glucosidic bond. And the disaccharide units are connected by β1→4 glucosidic bond to each other, forming the liner chain polysaccharides. The difference between CSA and CSC is the position of the sulfate groups. KS is composed of galactose and 6-sulfuric acid-N acetylgalactosamine, which are connected by β1→4 glucosidic bond, and one disaccharide are connected to another by β1→3 glucosidic bond , forming the liner chain polysaccharides. KS is the only glycosaminoglycan that dose not contain glycuronate. Besides its anti-neuralgia, anti-hemicrania and anti-tumor activities, SCPs was found to have some other functions, such as anti-rheumatoid...