Studies On Preventive Effects Of Sodium Ferulate,Chuanxiongzine,Panax Notoginseng Saponins,Ginkgo Biloba Extracts On Acute Oxygen Toxicity

When people inhale pure oxygen in excess of 3 atmospheres absolute (ATA)or higher, they may suffer from acute oxygen toxicity. With mainmanifestations as generalized, tonic-clonic seizures, acute oxygen toxicityis also defined as central nervous system (CNS) oxygen toxicity. In recentyears, the chances of breathing oxygen at higher pressures are more in suchactivities as military and professional diving, clinical treatment for moreand more sickness. In these courses, because of the poor tolerance tohyperbaric oxygen (HBO), or the fault of device and operation, CNSoxygen toxicity may occur. So it is very important to prevent this disease.Although prevention with medicines is an effective supplementary means,it is disappointed that by now, there are still no one satisfied, for theunnoticeable preventive effects or the adverse effects to the body,especially when administered for long period.In view of it is rare to prevent CNS oxygen toxicity with Chinesetraditional drugs, in our previous studies, we have tried and verified thatAngelica sinens is, Ligusticum chuanxiong, Panax notoginseng and Ginkgobiloba were more effective. On the base of these results, in our presentstudies, we investigated the preventive effects of Sodium ferulate (SF),Chuanxiongzine hydrochloride (TMP), Panax notoginseng saponines (PNS)and Ginkgo biloba extracts (GbE), the respective active composition ofthose drugs. We hypothesized that the preventive effects of thesecompositions were attribute to their antioxidative properties and theprotection of microcirculation. According to this hypothesis, the convulsionlatent periods and mortalities of mice exposed to 5 AlA 02 wereinvestigated. Besides, after exposed to 5 AlA 02 for 15 mm, thescavenging reactive oxygen species abilities of mice brain, the contents ofmalonic aldehyde (MDA), nitric oxide (NO) and glutathione (GSH), theactivities of catalase (CAT), glutathione peroxidase (GSH-Px) andmonoamine oxidase (MAO) in mice brain were determined. By means ofcytochemistry methods, we also observed the activities of alkaline3phosphatase (ALP) and Na+-K+-ATPase on the membrane ofmicrovascular endotheliocytes of mice cerebral cortex after mice wereexposed to 5 ATA O2 for 15 mm.Our results showed, SF, TMP, PNS and GbE could markedly postpone theseizures, prolong the convulsion latency and reduce the mortalities of miceafter they had convulsed. These compositions could also significantlyimprove the abilities of mice brain to scavenge the reactive oxygen species,cut down the contents of MDA, NO, elevate the levels of GSH and theGSH-Px activity. But they had almost no influences on CAT and MAOvitalities. Moreover, these compositions could obviously increase the ALPand Na+-K+-ATPase activities on the membrane of microvascularendotheliocytes of mice cerebral cortex. All these effects of theirs wereequal to that of VItE.In a word, through our experiments, we have demonstrated that the abilitiesof SF, TMIP, PNS and GbE to prevent acute oxygen toxicity were definite.We inferred the preventive effects of theirs were correlative to theirpowerful capabilities to scavenging oxygen free radicals and theirprotection for the transport functions of microvascular. With theseproperties, the compositions could enhance the antioxidative ability, ofbody and keep the stable internal environment that neuron exist.