The Study Of Neurokinin B Receptor In Mouse Nerve System

? IMMUNOHISTOCHEMICAL LOCALIZATION OF NEUROKININ B RECEPTOR (NK3) IN THE CENTRAL NERVOUS SYSTEM OF THE MOUSE AND ITS COLOCALIZATION WITH VASOPRESSIN (VPX OPIOD RECEPTOR (MOR) AND GABA1. IMMUNOHISTOCHEMICAL LOCALIZATION OF NEUROKININ B RECEPTOR (NK3) IN THE CENTRAL NERVOUS SYSTEM OF THE MOUSE AND ITS COLOCALIZATION WITH VASOPRESSIN ( VP ) objective To examine the localization of neurokinin B receptor (NK3)-like immunoreactivity (-LI) in the central nervous system of the mouse. Methods An immunohistochemical staining method was used. Results NK3 receptor-LI was localized in somatic and dendritic profiles in the most parts and in neuropil in a few regions of the mouse central nervous system. A large number of neurons with NIG-LI were seen in the anterior olfactory nuclei, accumbens nucleus, septal area,09600045, 39970377,39570109) 9ventral pallidum, pallidum, caudate putamen, nucleus of the stria terminalis, anterior hypothalamic area, tuber cinereum area, lateral hypothalamic area, perifornical nucleus, supraoptic nucleus, arcuate nucleus, mammillar nuclei, substatia nigra, ventral tegmental area, retrorubral area, superior and inferior colliculus, periaqueductal gray, nucleus of the solitary tract, and superficial layers of the medullary and spinal dorsal horns. The superfical layers of the cerebral cortex, piriform cortex, dorsal hippocampus, amygdal complex, reticular formation of the brainstem contained some neurons with NK3 receptor-LI. In the ventral hippocampus, median and intralaminar nuclei of the thalamus and interpeduncular nuclei, NKR-LI was localized in neuropil. Colocalization of NIG -LI and VP were studied in paraventricular nucleus and superoptic nuclei. The excited NK3 -containing neurons expressed FOS in major part neurons of paraventricular nucleus. Conclusion The results indicated that neurons with NIG receptor-LI were widely distributed in the central nervous system suggesting that it may be involved in many physiological functions in the mouse central nervous system. The colocalization results implicated that NK3 and vasopressin (VP) are in same neurons. And by regulating expressing and releasing of VP, NIG can modulate function of the paraventricular nucleus and superoptic nuclei. Especially it is closely related to salt and water taste.