| As the lifespan of human being prolonged, the aging event of human is being the focus of the society. Senescence and aging related diseases are the burden of the elderly, their relative and the society. Senescence of brain is the main factor, which has effects on the life quality of the elderly. Exploring the mechanism of brain senescence, looking for the effective therapy is the main task of the medicine. Modern western medicine has been in a controversy on the mechanism of senescence, and made no breakthrough in therapy. Traditional Chinese Medicine (TCM) has much originality of understanding brain senescence. Prof. Han Jingxian established the "YiQiTiaoXue, FuBenPeiYuan" acupuncture method in terms of the pathology of brain senescence, viz. deficiency of the kidney-essence, insufficiency of the spleen and stomach, blood stasis and stagnation of phlegm. The prescription consists of Shanzhong(Renl7), Zhongwan(Renl2), Qihai(Ren6), Xuehai(SplO), Zusanli(S36). The "YiQiTiaoXue, FuBenPeiYuan" acupuncture method can ameliorate the deficit of learning and memory in SAMP10, but the mechanisms of the therapy remain unknown. In present study, we used Senescence Accelerated Mouse (SAM) P10 and normal control SAMR1 as model, applied the cDNA Array technique, and monitored the expression difference of 588 genes in three group, viz.7-month SAMR1 control group, 7-month SAMP 10 control group, 7-month SAMP 10 "YiQiTiaoXue, FuBenPeiYuan" acupuncture method group to explore the mechanisms of brain senescence in SAMP10 and of antisenescence effect by "YiQiTiaoXue, FuBenPeiYuan" acupuncture method. The results showed:1. 53 genes' expression was different between the 7-month SAMR1 control group(SAMRl group) and 7-month SAMP 10 control group(SAMP10 group). 16 genes' expression was upregulated and 37 genes' expression downregulated in SAMP 10 group compared to SAMR1 group. The expression changed genes included:(l) Stress response proteins: the expression of Glutathione S-transferase 5, FGF-8; Fibroblast growth factor 8, NF-kappa-B transcription factor p65 subunit (NF-kB p65), heat shock 86-kDa protein( HSP86) and 84-kDa heat shock protein (HSP84) was upregulated and Cytochrome P450 1A1 downregulated in SAMP 10 group compared to SAMR1 group. (2) DNA synthesis, recombination, and repair proteins: the expression of DNA repairprotein RAD51 homolog 1(RAD51) and yeast DNA repair protein Rad52 homolog (Rad52) was upregulated and DNA excision repair protein ERCC5 and Recombination activating protein 1 (RAG1) downregulated in SAMP 10 group compared to SAMR1 group. (3) Neurotrophic factors and receptors: the expression of Insulin-like growth factor I receptor alpha subunit was upregulated and Insulin-like growth factor II precursor, Insulin-like growth factor-IA, Growth hormone receptor and Estrogen receptor downregulated in SAMP 10 group compared to SAMR1 group. (4)Apoptosis associated proteins: The expression of FLICE-like inhibitory protein long form and Apolipoprotein J was upregulated and BAX membrane isoform alpha, Protein kinase B and Lymphotoxin receptor downregulated in SAMP 10 group compared to SAMR1 group. (5) Synapse related proteins: The expression ofcAMP-dependent protein kinase type I-beta regulatory chain and 14-3-3 protein eta was upregulated and Retinoic acid receptor beta-2, ERBB-2 receptor, PSD-95/SAP90A and Neural cadherin precursor downregulated in SAMP 10 group compared to SAMR1 group.(6)Cell adhesion receptors-proteins: The expression of Leukocyte adhesion glycoprotein LFA-1 alpha chain was upregulated and Cdl4, Cell surface glycoprotein mac-1 alpha subunit and Integrin alpha 4 downregulated in SAMP 10 group compared to SAMR1 group. (7) Cell receptors: The expression ofInterleukin-7 receptor alpha was upregulated and 5-HT receptor IB receptor and Macrophage colony stimulating factor 1 (CSF-1) receptor downregulated in SAMP10 group compared to SAMR1 group.(8) Cell signaling: The expression of Bone morphogenetic protein 4 precursor (BMP4), Placental ribonuclease inhibitor, angiogenin, Vascular endothelial growth...
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