Study Of Blood CK After Exercise In Relation To Exercise-induced Muscle Damage

It will be of great significance not only to successful arrangement of proper training load for athletes, but also to reducing exercise-induced injury and maintaining of potential physical performance and health condition if exercise-induced muscle damage could be evaluated precisely just in time by changes of serum CK and isoenzymes. So the aim of this study is to probe the correlation between exercise-induced muscle damage and changes of serum CK and isoenzymes after exercise by animal and human experiment, with the effects of different nutritious tonic on these changes investigated at the same time. The study is consisted of the following four parts.1st experiment. Relationship between changes of rat plasma CK activity after progressive load swimming exercise and muscle damage. 60 male Wistar rats were divided into groups randomly. Rats of training group undertook a 6-week swimming training program with progressively increasing load, with an exhaustive swimming exercise at last. Untrained rats were divided into 25-minute group, 50-minute group and exhaustive group in according to swimming duration. Exercise-induced muscle damage was reflected by Z-band abnormality of rat soleus muscle, with changes of serum and red gastrocnemius muscle CK measured simultaneously. Results found, there was a significant correlation between Z-band abnormality of soleus muscle and changes of plasma CK and CK-MM of untrained rats after swimming, with coefficients being r=0.976(P<0.024) and r=0.981(P<0.019). The Z-band abnormality and level of plasma CK and isoenzymes of trained rats after swimming was lower (P<0.1) than that of untrained rats. It was concluded, 〢n inseparable correlation between exercise-induced muscle damage and plasma CK, CK-MM of untrained rats after swimming was found. (2)The degree of exercise-induced muscle damage was significantly reduced by swimming training, with changes of plasma CK also decreased.2nd experiment. Changes of plasma CK activity of down hill running trained rats after eccentric exercise with different load and exercise-induced muscle damage. 40 male Wistar rats were divided into groups randomly. Rats of training groups undertook a 2-week down hill running program with progressively increasing load, an exercise of heavy, moderate and light load of down hill running would be performed at last. Results found, the coefficients between Z-band abnormality of soleus muscle and plasma CK and CK-MM of trained rats after down hill running with different load were r=0.998(P<0.002) and r=0.99(P<0.01). The plasma CK-MM activity was significantly lower (P<0.05) after 48 hour's recovery than that of immediately after exercise. These results indicated, ㏕here was a significant correlationbetween exercise-induced muscle damage and plasma CK and CK-MM of down hill training rats after eccentric exercise. (2)Changes of plasma CK and isoenzymes of eccentric exercise trained rats primarily recovered 48 hours after exercise.3rd experiment. Changes of serum Mb, LDH, CK and isoenzymes of male and female judo players after heavy load exercise. 5 male and 4 female judo players, averaged 16.3 ± 0.3 and 16.2±0.5 years old. Blood was taken from vein at before exercise and Oh, 12h, 24h, 36h and 48h after exercise with Mb, LDH, cTNI, CK and isoforms measured. Results found, (1) Serum CK-MM 3 of male players at 24h after exercise was significantly greater(P<0.05) than the level before exercise. (2)Peak value of serum Mb of male players appeared immediately after heavy exercise, and was significantly greater(P<0.05) than the level before and 12h after exercise. (3)There was no significant changes in serum Mb, CK and isoforms of female players after heavy exercise compared with male players.4th experiment. Changes of serum Mb, LDH, CK and isoforms of basketball players after heavy load exercise. 11 male basketball players, averaged 21.8±1.0 years old. Results found, peak of serum CK and CK-MM of players appeared at 24h after exercise. While peak value of serum Mb, CK-MM3% and CK-MM3%/CK-MM]% was significan...