Rhaponticum Uniflorum (L.) DC. Takes Out Tomention The Dose Tumor Multidrug Resistance Turning And Inducing Apoptosis

This paper with animal transplant tumour H22 liver cancer and S180 sarcoma is model, observe the Rhaponticum uniflorum(L.) DC. Chinese medicine mellow wine take out to mention dose (RHU) different density as well as with common melt Liao medicine obey platinum and ring Lin Xian amine suit use or contrast utilize for lotus tumour animal restrain tumour and synergism increase quick , strengthen organism immunity force , prolong the curative effect of survival issue role. Detection not organizes little mouse weight change and tumour weight together with medicine , lung body than , spleen body than , liver body than , chest gland body than. With the person MCF-7 sensitive of breast cancer with endure medicine MCF-7/ADR is research object, difference is tested with cell poison ( MTT law ) , flow cytometry (FCM) with place upside down to differ microscope, detection not withers together with medicine group body outside training cell to die , the change of BcL-2, end grain enzyme and the etc. of P-gp ( P170 proteide ) with express condition. Show as a result: RHU take out to mention dose have certain restrain tumour role have obvious quantity just effect relation; The immunity force of protective organism increases weight , is obvious to prolong the survival time of experiment lotus tumour animal. With the coordination of Western medicine use, can develop obvious synergism , increase quick , reduce poisonous role; Can express apparently under tune MDR/P-gp, telomerase and BcL-2 gene , also present dosage-the relation of curative effect. Thus worse turning endures medicineand guides to wither, is deceased to take affect. Mechanism is its role:1. RHU role in the tumor cell, issue of G2/M, retardance tumor cell has silk to split;2. RHU can hold grain enzyme apparently under tune tumor cell express , it is deceased to guide tumor cell to wither;3. RHU influence tumor cell gene express , can promote cancer growth gene bcl-2 under tune to express;4. RHU can restrain tumor cell apparently endure medicine gene MDR/P-gp express , it is active to restrain to endure medicine cell membrane transfer, decrease to endure medicine cell for melt Liao the outside row of medicine, raise cell melt Liao medicine density, strengthen for cancer cell kill and wound force, so worsen tumor cell endure property of medicine;5. RHU have fight bad ill quality role efficiently, the immunity force of protective organism increases weight , is obvious to prolong the survival time of experiment lotus tumour animal.Above research work can settle heat for further development application detoxify medicine leakage Lu application in restrain tumour, worse turning endures medicine , guide tumor cell wither deceased aspect offer important reference basis.