| PrefacePercutaneous transluminal coronary angioplasty (PTCA) is an important breakthrough of the therapy of cardio vascular disease, but the high incidence rate of the restenosis (RS) influence the distant effect obviously. At present, we consider the major mechanism of RS refers to the elastic contraction of extended artery segment, migration and proliferation of vascular smooth muscle cell (VSMC ). production of extracellular matric, remoulding of vascular wall and participation and organization of thrombosis . During the process of RS, all kinds of active substance and growth factor act by special receptor, produce transcript factor by intracellular signal transduction, promote special intranuclear gene expression and produce biological effect. Mitogen-activated protein kinase (MAPK) is common passage of intracellular messenge transduction to promote cellular proliferatian and key enzyme of cell phenotype change and proliferation. Protein kinase C ( PKC) is an important passage that extracellular signal lead to short -term biological effect in cytoplasma. Moreover, PKC plays an important role in nuclear reaction. As upper reaches signal molecule, PKC can active MAPK. Vascular endothelial growth factor ( VEGF) is a special motivating division agent of endothelial cell, but cant motivate division of VSMC fibrocyte. VEGF may play an important role in prevention and treatment of RS.TongXinLuo (TXL) is studied by Hebei Yiling Medicine Group. It has been used to treat coronary heart disease. This trial makes artery iniury model of rats and uses TXL to intervene treatment and proves that TXL can inhibit endo-membrane proliferation and RS in biological standard.Materials and Methods1. Trial animal; 105 healthy male Wistar rats, weight 300-350g, week -age 14-16 weeks, provided by CMU trial animal department.2. Construction of artery saccule injury model: 105 healthy male Wistar rats, weight 300 ~ 350 g. Making artery saccule plastic operation model by De-man or other methods. Inserting 2F Fogarty saccule catheter made by self, in thoracic aorta through femoral artery and injury and injury dilated abdominal aorta.3. Trial aninal group: Group of saeccule injury (injury group) 45 rats, each 15 rats of postoperation 3d,7d and 14d . Group of using medicine after saccule injury (therapy group) 45 rats, each 15 rats of 3d,7d and 14d. Non-in-jnry group (normal contrast group) 15 rats.4. Executing animal and taking material: weighting animal, abdominal anaesthesia by pentobarbital natrium, perfusion by heparin and physiological saline. Take 3. 0 cm abdominal aorta and divide into three segment and put in 10% formaldehyde solution, 2.5% pentodialdehyde fixation liquid nitrogen jar freezing and then conserve in-70℃ deep freezzing refrigerator.5. Making pathologic specimen:(1) Photoscope specimen; 10% formaldehyde solution fixation, paraffin embedding, continue freezing section, HE staining.(2) Electroscope specimen: 2.5% pentodialdehyde fixation, 1% osmium acid post - fixation, Epon 812 saturation embedding ultrathin section, uraninm and aluminium staining.6. Measurment of Vascular tissue MAPK activity: Use MBP as substrate, measure MAPK actvity by r - 32P ATP in vitra marking phospheration.7. Measurment of vascular tissue PKC activity: Use histon as substrate, measure PKC activity by r - 32P ATP in vitra marking phospheration.8. Western Blot measuring protein expression of ERK1, ERK2, PKCa, VEGF. Use special antibody of ERK1, ERK2, PKCa, VEGF, measure proteinexpression of ERK1, ERK2, PKC2, VEGF in vascular tissue, protein extract fluid.9. RT-PCR measure ERK1 ERK2, PKCa, VEGF gene expression; Use Trizol summary RNA extract reagent, extract summary RNA of testis tissue and reverse-transcript and synthesis cDNA 1st chain, expand PCR outcome of ERK1, ERK2, PKCa, VEGF partly and measure mRNA content.Results1. Vascular morphology changes: In nonnal contrast group, abdominal aorta endothelium of rats is complete. At 3rd day after saccule injury, end...
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