Effects Of NF-κB On Rats Liver Regeneration And Apoptosis After 70% Portal Branch Ligation

Portal branch ligation or embolization already have applied extensively for liver carcinoma, especially for the advanced patients who had already missed the opportunity and have already gotten fairly good effects, furthermore some patients get the chance for tumour resection on the second stage. Portal branch ligation or embolization could produce atrophy of the deprived lobes, whereas the perfused lobes undergoes compensatory regeneration, the liver structure and function maintain normal. The mechanism is still unclear. It is demonstrated that NF-кB and some cytokines (for example TNF-αand IL-6) modulated by NF-кB have important roles on cell regeneration and apoptosis. To study weather they have effects on liver regeneration and apoptosis after portal branch ligation, we observed the changes of DNA binding activity of NF-кB and the expression of TNF-α and IL-6 and the relations to hepatocyte regeneration and apoptosis after 70% portal branch ligation .PART ONEObjective To observe the effects of 70% portal branch ligation on rats liver structure and function. Methods Sixty Wistar rats were randomized divided into the control group and the portal branch ligation group. The animals were killed on the 12th hour, 1st, 2nd, 3rd, 7th and 14th day respectively. To determine the contents of plasma ALT and AST. The microstructure changes of liver were observed by microscope. The ultrastructural changes of the deprived lobes were observed by electron microscope. Results After 70% portal branch ligation, which producedatrophy of the deprived lobes, whereas the perfused lobes underwent compensatory regeneration. The total liver weight and plasma ALT and AST levels were maintained at the level of sham-operated animals throughout the experiment. There were only slight necrosis in the deprived lobes and evident regeneration changes in the perfused lobes on the early stage. There were typical apoptotic changes on early stage and evident fibrotic changes on advanced stage in the derived lobes under electron microscope. Conclusion 70% portal branch ligation could produce atrophy of the deprived lobes, whereas the perfused lobes undergo compensatory regeneration, the total liver weight and function maintain normal.PART TWOObjective To observe the changes of rats hepatocytes apoptosis and regeneration after 70% portal branch ligation. Methods Sixty Wistar rats were randomized divided into the control group and the portal branch ligation group. The animals were killed on the 12th hour, 1st, 2nd, 3rd, 7th and 14th day respectively. The degree of hepatic regeneration and apoptosis were observed by microscope and TUNEL. Hepatocytes were isolated and DNA was extracted for gel electrophoresis. The ultrastructural changes of the deprived lobes were observed by electron microscope. Results After 70% portal branch ligation, which produced atrophy of the deprived lobes, whereas the perfused lobes underwent compensatory regeneration. On the 2nd day, the number of apoptotic hepatocytes of the deprived lobes and the number of mitotic hepatocytes of the perfused lobes both reached peak. There were typical DNA ladder of gel electrophoresis on the deprived lobes. There were typical apoptotic changes on early stage and evident fibrotic changes on advanced stage in the derived lobes under electron microscope. Conclusion 70% portal branch ligation could produce atrophy of the deprived lobes through hepatocytes apoptosis, whereas the perfused lobes undergo compensatory regeneration through hepatocytes mitosis.PART THREEObjective To study the effects of NF-кB on rats liver regeneration and apoptosis after 70% portal branch ligation. Methods Sixty Wistar rats were randomized divided into the control group and the portal branch ligation group. The animals were killed on the 12th hour, 1st, 2nd, 3rd, 7th and 14th day respectively. Hepatocytes were isolated and nuclear protein was extracted, DNA binding activity of NF-кB was measured by EMSA. Results After 70% portal branch ligation, DNA bi...