| OBJECTIVE: To study liver regeneration of the nonligated liver lobes following portalbranch ligation.METHODS: Sixty male healthy Wistar rats(weight 250 ?30g) wererandomly divided into experimental group and control group and were subjected to portal branch ligation (PBL) and sham operation, respectively, following ether anaesthesia.The animals were killed following ether anaesthesia on the lst,2nd,3rd,7th and 14th day respectively. The blood was collected from heart and the livers were harvested to examine Serum alanine aminotransferase(ALT) levels and total liver weight,respectively.The pathological changes of hepatic histology were observed through light microscope. The number of liver cell nuclear mitosis index was counted. The number of proliferative cell nuclear antigen (PCNA) index was counted by immunohistochemistry method. The ultrastructural changes of liver were observedunder electron microscope.RESULTS: (1) Serum ALT levels that were back to normal verysoon showed an increase change in first day after operation in PBL groups compared with control groups(p<0.01).(2) The total liver weight showed no significant change between PBL groups and control groups (p>0.05).(3)Liver cell nuclear mitosis index was markedly increased in PBL groups compared with control groups(p<0.01).(4) Compared with sham operation groups, PCNA indexwas significantly increased in PBL groups(p<0.01).CONCLUSIONS:1.The left portalbranch ligation can induce active regeneration of hepatic cell of nonligated liver lobes in rats. The liver may restore previous weight following regeneration.2.The portal branch ligation of 75% live lobes does not affect liver function and may be safely performed.3.The rat of portal branch ligation may regard as a model animal of liver regeneration for research. |
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