Study On The Expression Of β-defensin-2, Tumor-necrosis-factor-alpha And Nuclear-factor-κB P65 In The Lesions Of Psoriasis Vulgaris

Objective (1) To investigate the expressions ofβ-defensin-2(HBD-2), tumor-necrosis-factor-alpha(TNF-a), nuclear-factor-kBp65(NF-kBp65) in lesions of psoriasis vulgaris; To study the correlation between their expressions intensity and disease severity. (2) To confer on their effects on the occurrence and the development of psoriasis.Methods (1) Specimens were collected from 45 patients with psoriasis and 15 normal human controls. The expressions of (3-defensin-2, tumor-necrosis-factor-alpha, nuclear-factor-кB p65 in the lesion organizations of 45 psoriasis patients,the non-lesional tissues of 45 patients with psoriasis and 15 normal skin samples were measured by SP immunohistochemical method.The comparison and the correlation ofβ-defensin-2, tumor-necrosis-factor-alpha and nuclear-factor-KBp65 expression in different groups. (2) Psoriasis disease activity was evaluated by PASI (Psoriasis Area and Severity Index). According to PASI scores, the patients were divided into three groups:psoriasis light 25, moderate 12 and severe 8. To investigateβ-defensin-2, tumor-necrosis-factor-alpha and nuclear-factor-KBp65 expression.The comparison and the correlation analysis of theβ-defensin-2, tumor-necrosis-factor-alpha, nuclear-factor-KB p65 expression in these three groups.Results (1) Relative to the normal skin tissues and the non-lesion areas of psoriasis, theβ-defensin-2, tumor-necrosis-factor-alpha and nuclear-factor-KBp65 expression remarkably went up in psoriatic lesion areas,and the tumor-necrosis-factor-alpha andβ-defensin-2 expression in non-lesion areas were higher than that in normal skin tissues as well, and the differences were all statistically significant (P<0.05); however, when comparing the non-lesion areas with the normal skin tissues in nuclear-factor-KBp65 expression, the difference was not statistically significant (P> 0.05). In the normal skin tissues, the non-lesion areas of psoriasis and the psoriatic lesion areas, there were all positive correlations between theβ-defensin-2 and tumor-necrosis-factor-alpha,tumor-necrosis-factor-alpha and nuclear-factor-кBp65,β-defensin-2 expression and nuclear-factor-KBp65 expression level (P<0.05). (2)In psoriatic lesion areas, with the comparison ofβ-defensin-2, tumor-necrosis-factor-alpha and nuclear-factor-KBp65 expressions in the mild,moderate and severe group, the three indexes'expression in the severe group were higher than moderate group, which is higher than mild group. The differences were all statistically significant (P<0.05).Conclusion:(1) HBD-2 and TNF-aexpressions in normal skin tissues, psoriatic non-lesional areas and lesional areas gradually increased, and NF-кB p65 in psoriatic lesion areas was expressed significantly. (2) HBD-2, TNF-a and NF-кB p65 expressions in lesions of psoriasis vulgaris obviously increased, and the worse the lesions were, the more the expressions were. There were positive correlations between HBD-2 and TNF-a, TNF-a and HBD-2, HBD-2 and NF-кB p65.In a word, HBD-2, TNF-a and NF-кB p65 are closely related to the occurrence and development of psoriasis vulgaris. There may be mutually promotive effects for the three indexes on the development of psoriasis so that the lesions are degenerated.