Study On Differentially Expressed Genes In Postburn Hypertrophic Scar With CDNA Microarray

Any wound healing is accompanied by various scars, which can also be looked as healed signal in surgery. The formation of a hypertrophic scar is an abnormal wound-healing response after thermal or partial-thickness injuries. The excessive aggradation of collagen and repair in excess could induce excessive proliferation and abnormal contracture in scar, both of which which also destroy the superficial integrality of body and cause various malfunction. The injury of mammalian skin leads to a complex series of cellular and molecular events, as cells respond to trauma and attempt to repair damaged regions. It is found that different species, human races, individuals and even different body positions suffered from scar differently. As plenty of research reported, there are complicated cell movement and substance metabolization in either actively proliferating or mature scars. Further studies have implicated that extracellular matrix molecules, cytokines and proteinases might alter cell proliferation and migration behavior in the formation of hypertrophic scar. However, the understanding of formating process of excessive scar have been limited until now in spite of several centuries study. For reason of unclear pathogenesis, it still could not be controlled with current methods and thus satisfying result.Along with the rapid development of cellular and molecular biology in recent years, the researches which reveal the mechanism of wound healing andscar formation have made great progress. The molecular foundation of scar formation involved in growth factors and extracellular matrix molecule, signal transduction, cell cycle control, synthesis of proteins, apoptosis and so on. It has many common grounds with collagenous disease and tumor occurrence. On the view of modern biology, any change in response to an injury must be accompanied by a change in gene expression. It is known that the mutation or change of genes maybe cause the tissue or organs to tumor occurrence, which is a gene disease in some way. According to this, the formation of hypertrophic scar should have great deal of genes changed. But we know still little about what and how these lead hypertrophic scar occurrence.There are many deficiencies in traditional molecular biologic method, such as Northern Blot, hybridization in situ, which can merely analyze one or several genes synchronously and need long time as well as more samples. RT-PCR can not analyze difficult diseases thoroughly with its own restrictions, though more sensitive and more genes analyzed. A broadscale evaluation of differential gene expression in the formation of hypertrophic scars may be valuable for finding specially scar related genes. More detail study on these genes may provide a new way to control hypertrophic scar.The human Genome Initiative has motivated researchers to develop different technologies that exploit genomic sequence information to assess clinical disorders. With lots of gene signals in localized carrier, microarray can detect thousands of genes expression under nearly consistent conditions with limited samples quickly. On such example, cDNA microarry analysis, allows the simultaneous comparison of thousands of genes. The recent development of microarry analysis has enabled us to compare global gene profiling from various normal and affected samples. The analysis by gene chip made a great deal of valuable achievements in many normal and carcinoma tissue expression research. The comparison of gene profiling of normal skin and hypertrophicscar may identify possible mRNA transcripts that are responsible for the formation of scar and therefore lead to potential therapeutics or prevention.We compared differentially expressed genes between normal skin andhypertrophic scar from 4 patients with microarry containing 4096 human genes.When these genes were analyzed, we could better understand their roles in theformation scar. A group of genes belonged to cytoskeleton and movement withsimilar functions were selected and studied their express...