The Theory Study On Hemi-sectioned Spinal Cord Injury Machanism And Recovered In Rats

A Hemi-sectioned spinal cord injury (hSCI) manifests itself in sequard's syndrome .In order to explore to its pathogenesis and recovery process and to work out the relationship between its pathological ,bilochemical, molecule and genetic changes,the present study proposed to make a model for the hSCI on the basis of the descriptions made by professor XU Shaoting:made hemi-section of the 5th to 7th section of the cervical spinal cord and the 8th to 11th section of the thoracic spinal cord(the scalpel was inserted into the central crevice of the spinal cord.Then it went from the central tube to the front crevice, and cut horizontally in the firrst place.Half of the horizontally cut part was taken as the research object).After the operation.the tissue changes were subjected to observations at different time,and the obs were recordedaccordingly.Findings:Degeneration and necrosis of the far end of the lateral cord and the nerves of the substance grisaille of the hemi-sectioned part of spinal cord were detected by light microscope was of sognificance to make assessment for different study group and sham-operated group at 24 hours and 1 week after the operation(P<0.05).further ,the ventral horn's motor neuron group,the fact of the rat.Conclusion:Since the model of the hemi-sectioned cervical spinal cord was follow by a low mortality rat,it was able to be regarded as a reliable apporach.The present experiment adopted an immunohistochemical detecting method to sort out the changing distribution of the endothelin-1 at different point of time after the injury appeared. An image pattern analysis was also employed to provide a semi-quantitative account of the quantity of the endothelin-1 at the far and near end of the injury.The density of the method of atomic spectrum absorption and image pattern analysis at 1,4,8,24,72 and 168 hours after the hSCi occurred .The changing distribution of protein s-100 in verve tissue at various stages of the Brown-Sequard's syndrome was observed by means of immunohistochemical technique.Examination of the amount and position of apoptosis cells at different stage of the injury was carried out by way of 1 SEL method,and normal contrasts were to be made.The significance of the present study.The relationship between the activity of ALT and AST in the injured rat's serum and the injury was put under examine to evaluate the conection between the changes of the activity of ALT and AST and the seriousness of injury.In addition the functional role of protein P53 in the hSCI was discussed by examining the spinal tissue at 30 minutes 2,4,8,24,48,72 hours,and 6 weeks after the injury occurred.The experiment made use of acouple of widely employed medications in clinical practice,GM-l and methylpredniosone,to treat the rat for a hSCI,and tried to judge the influences of methylpredniosone on the genetic expression of c-fos after injury by examining gene as well as to evalute the therapeutic effect of GM-1 .AT 1 ,6,24 hours and l,6week after the injury occurred ,the injuryed tissue of the cervical spinal cord of the injury occurred,the injured group and the treating group respectively was submitted to the examination by a light or electron microscope.Then ,a Rivlin experiment was carried out to test the rat's motor function.Before and after medication was given,the pathogenesis.the blood in the substance grisaille,motor evoked potential(MEPs),regular patterns of motor changes and their interrelations had been examineed.At different point of time,the motor function of the rat,the MEPs,the blood in in the substance grisalle of the injured part were tested while the changes under the light or electron microscope were paid attention to.. To observe the effect of olfactory ensheathing cells(OECs) transplantation on spinal cord axonal regeneration and spinal functional improve rats.OECs suspen sion,Purified from SD rat olfactory bulb,was transplanted into the left semi-sectioned spinal cord at C6 level,while the vehicle solution the rats were evaluated with climbing test and MEP...